Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue |
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Authors: | S Kirkeby |
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Institution: | Institute of Anatomy, Royal Dental College, Copenhagen Ø, Denmark |
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Abstract: | In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained. |
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