| 石蜡包埋大肠癌肿瘤浸润淋巴细胞FCM单细胞悬液样本的制备 |
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| 引用本文： | 石磊,杨伟明,赵洪远,骆礼波,贺新媛,任宇,苏晓玥,刘勤,陈梦婷,霍金龙.石蜡包埋大肠癌肿瘤浸润淋巴细胞FCM单细胞悬液样本的制备[J].现代保健,2014(6):19-20. |
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| 作者姓名： | 石磊 杨伟明 赵洪远 骆礼波 贺新媛 任宇 苏晓玥 刘勤 陈梦婷 霍金龙 |
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| 作者单位： | 遵义医学院附属医院,贵州遵义563099 |
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| 基金项目： | 贵州省优秀科技教育人才省长专项资金资助项目（200779） |
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| 摘 要： | 探讨石蜡标本中大肠癌肿瘤浸润淋巴细胞亚群单细胞悬液样本的制备方法。石蜡标本经切片、脱蜡、水化、消化、过滤、收集细胞等步骤制成单细胞悬液,滴加单克隆抗体,通过流式细胞仪检测,最后进行淋巴细胞亚群细胞分型获取及读数。
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| 关 键 词： | 大肠癌 肿瘤浸润淋巴细胞 石蜡包埋 流式细胞术 |
Making the FCM Checking Tumor Infiltrating Lymphocytes in Colorectal Carcinoma into Single Cell Suspension |
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| Institution: | SHI Lei;YANG Wei-ming;ZHAO Hong-yuan( The Affiliated Hospital of Zunyi Medical College, Zunyi 563099, China) |
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| Abstract: | To explore the ways of making the FCM checking tumor infiltrating lymphocytes in colorectal carcinoma into single cell suspension.The collection was made into single cell suspension by sliced,deparaffinage,hydration,digestion,filtration and cells collection,adding monoclonal antibody and checking by flow cytometer,getting lymphocyte subpopulations and fetching the dates. |
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| Keywords: | Colorectal carcinoma Tumor infiltrating lymphocytes Paraffin-embedded Flow cytometry |
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