黄芪多糖对链唑脲霉素诱导的2型糖尿病大鼠肾脏TGF-β_1表达的影响

目的:探讨黄芪多糖(APS)对链唑脲霉素(STZ)诱导的2型糖尿病(T2DM)大鼠早期肾脏病理改变的治疗作用及其机制。方法:雄性SPF级SD大鼠随机分为正常对照组(C组)、APS对照组(CA组)、T2DM组(DM组)及APS治疗组(DA组),每组8只。观察治疗前后各组大鼠血糖、葡萄糖耐量变化,肾脏组织形态学和转化生长因子β1(TGF-β1)表达等的变化。结果:DM组大鼠血糖显著高于C组,同时伴有明显的糖耐量异常(P<0.01),经APS治疗8周后,DA组血糖降低,糖耐量改善(P<0.01)。DM组大鼠的肾小球面积较正常大鼠大、系膜基质增加,肾小管管腔扩大,管壁变薄,肾小球基底膜(GBM)增厚,可见蛋白管型,APS治疗可减轻T2DM大鼠肾脏病理改变,降低TGF-β1的表达水平。结论:APS能够降低血糖,增强机体胰岛素敏感性,减轻DM肾脏病理损害。其机制与APS降低T2DM大鼠肾脏TGF-β1表达有关。

Effect of APS on the Expression of Renal TGF-β1 in STZ Induced Type2 Diabetic Rats

Objective: To investigate the potential effect and possible mechanism of Astragalus polysaccharide (APS) on the early pathological changes in kidney of type 2 diabetic rats.Method: Male SD rats(SPF) were randomized to four groups: control group(C), APS control group(CA), T2DM group (DM) and APS treated group (DA) , 8 rats in each group.Blood glucose and oral glucose tolerance test were observed pre-and post APS therapy in all groups.Results: Compared with C group, there was a significant increase in blood glucose level and a marked impairence of oral glucose tolerance test in DM group (P<0.01) ,which was alleviated by treatment with APS for 8 weeks(P<0.01).While the above changes didn't occur in normal SD rats treated with APS. In DM group's rats, renal glomerulus surface was magnified, mesenterium basilaris substantia was thicker, urinary cast lumina was enlarged, vessel wall was thinner,GBM was thickened and obvious. TGF-β1 staining was positive.APS treatment alleviated these pathological changes.Conclusion: APS treatment ameliorates insulin resistance,decreasing the level of blood sugar and improving oral glucose tolerance. It has a protective effect on kidney of T2DM rats, which is partly related to reducing expression of TGF-β1.