人脐带间充质干细胞不同部位移植对压力性尿失禁治疗作用的研究

目的研究尿道周围及坐骨神经近端移植人脐带间充质干细胞（hUCMSCs）是否可以提高压力性尿失禁大鼠的尿道闭合压。改善其储尿能力。方法模拟绝经和产伤构建压力性尿失禁大鼠模型，并用漏尿点压力（LPP）、最大膀胱容积（MCC）及“模拟喷嚏”实验进行检测。将hUCMSCs分别注射移植到压力性尿失禁大鼠的尿道周围及坐骨神经近端。1个月后再次检测漏尿点压力及最大膀胱容积。结果移植后1个月，尿道周围治疗组与尿道周围模型组MCC差值差异无统计学意义（P〉0．05）；而尿道周围治疗组LPP增高，尿道周围模型组LPP降低，两组差值差异有统计学意义（P〈0．01）。移植后1个月．坐骨神经治疗组MCC和LPP与坐骨神经模型组相比，其差值差异均无统计学意义（P值均〉0．05）。结论人脐带间充质干细胞注射移植到尿道周围可以提高压力性尿失禁大鼠的尿道闭合压，但不能改善其储尿能力；而移植到坐骨神经近端短期内不能改善压力性尿失禁大鼠的储尿能力。

The efficiency of SUI treatment by transplanting hUCMSC in different position

Objective To determine whether the injection of human umbilical cord mesenchymal stem cells （hUCMSCs） in periurethral and the sciatic nerve proxima could increase urethral closure pressure and improve urine storage capacity of stress urinary incontinence in stress urinary incontinence rats. Methods Simulate menopause and birth trauma to build SUI rat and the model was further examined by leak point pressure （LPP）, maximum bladder capacity （MCC） and ＂simulated sneeze＂ experiment. The hUCMSCs were injected into the sciatic nerve proxima and periurethral. LPP and MCC were examined 1 month after transplantion. Results Compared to periurethral model group, the difference value of MCC was not statistically significant in periurethral treatment group, 1 month post-transplantation （P〉0.05） ; LPP increased in periurethral treatment groups but failed in model group, the difference was statistically significant （P 〈0.01 ）. 1 month after transplantation, the differences of MCC and LPP were not statistically significant （P〉0.05） between sciatic nerve treatment group and sciatic nerve model group. Conclusion Transplanting hUCMSCs around the urethra can improve urethral closure pressure but not urine storage capacity. Transplanting hUCMSCs into the sciatic nerve proximal in SUI rats does not improve urine storage capacity within short term.