ET-1促进人血管平滑肌细胞的表型变化和增殖

目的：研究ET-1与VSMC表型变化和增殖的关系。方法：用MTT法研究ET—1对平滑肌细胞增殖的影响，^3H-TdR法测定ET-1对平滑肌细胞DNA合成的作用，流式细胞仪法观察对平滑肌细胞增殖周期的影响，逆转录聚合酶链法观察对平滑肌细胞表型变化的影响。结果：与对照组比较，ET-1明显促进平滑肌细胞增殖；促进平滑肌细胞DNA合成；促进平滑肌细胞从G0期向S期的转变，G0／G1期细胞百分比明显降低，而S期细胞百分比增加；促进平滑肌细胞的表型转化，从第2d到第7d随时间延长1-Caldesmon表达逐渐增高。结论：ET-1促进平滑肌细胞表型转化，同时对平滑肌细胞增殖亦有明显的促进作用。

Endothelin-1 stimulates phenotype and proliferation of human umbilical artery smooth muscle cells

Objective: To study the effects of endothelin-1 (ET-1) on phenotype and proliferation of culture human umbilical artery smooth muscle cells (HUASMC). Methods: The effects of endothelin-1 on HUASMC were evaluated by MTT assay. The content of DNA was observed by 3H-TdR assays and cell cycle was analyzed by flow cytomerty. RT-PCR method was employed to detect the effect on HUASMC phenotype. Results : ET-1 (100 nmol/L) stimulated HUASMC proliferation compared with the group without ET-1. ET-1 stimulated DNA synthesis of HUASMC compared with the group without ET-1. ET-1 promoted the cells cycle transition from Gb/Gi phase to S phase. Go/Gj phase cells percentage obviously decreased compared with the group without ET-1. S phase cell increased compared with the group without ET-1. Furthermore, it triggered the expression of 1-caldesmon. Conclusion: ET-I may stimulate phenotype and proliferation of HUASMC.