携细胞间黏附分子1抗体的Sono Vue微泡造影剂靶向识别受损内皮的实验研究

目的 探讨静电吸附法制备的携细胞间黏附分子1抗体靶向微泡在体外和体内的寻靶能力.方法 采用静电吸附法制备携ICAM-1抗体的靶向SonoVue微泡.体外培养经IL-1β刺激大量表达ICAM-1的人血管内皮细胞ECV304,采用免疫荧光法检测靶向微泡与其结合能力.构建兔急性心肌梗死模型,进行靶向微泡的心肌造影,采用冷冻切片和免疫荧光法榆测靶向微泡与受损血管内膜结合能力.结果 在体外实验中,可见大量携ICAM-1抗体靶向微泡与刺激后ECV304细胞紧密结合,仅有少量靶向微泡与正常ECV304细胞结合.体内实验中,可见大量携ICAM-1抗体靶向微泡在兔梗死心肌受损血管内膜处黏附,仅可见少量靶向微泡在正常血管内膜处黏附.结论 携ICAM-1抗体靶向微泡在体内、体外均能与受损血管内皮特异性结合,不仅有利于靶向超声造影显像,更为微泡携带药物或基因在局部定向释放开辟良好前景.

Experimental study on detecting damaged endothelium with the targeted mirobubbles carrying ICAM-1 antibody

Objective To discuss the feasibility of targeted microbubbles conjugated to intercellular adhesion molecule-1(ICAM-1)antibody in evaluating damaged endothelium.Methods Targeted microbubbles carrying ICAM-1 antibody was prepared by the electrostatic attraction way.Targeted microbubbles carrying ICAM-1 antibody was added to activated ECV304 in vitro.In vivo,the target ability of targeted microbubble to the damaged endothelium was detected.Results The results of homing test in vitro showed that the targeted microbubbles carrying ICAM-1 antibody could be combined with ECV-304 stimulated by IL-1β specifically.The targeted microbubbles carrying antibody retained within the damaged endothelium were much more than the standard endothelium.Conclusions Mierobubbles conjugated to ICAM-1 have been shown to bind to damaged endothelial cell.