| Localization of NKCC1 in the Cochlea and Morphology of the Cochlea in NKCC1-Knockout Mice |
| |
| 作者姓名： | 褚汉启 熊浩 周小琴 黄孝文 周良强 崔永华 |
| |
| 作者单位： | Department of Otolaryngology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China，Department of Otolaryngology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China，Department of Respiratory Disease Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China，Department of Otolaryngology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China，Department of Otolaryngology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China，Department of Otolaryngology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China |
| |
| 基金项目： | ThisprojectwassupportedbygrantsfromNationalNatu-ralSciencesFoundation(No.30371526)andHubeiProvin-cialNaturalSciencesFoundation(No.2002AB127)ofChi-na. |
| |
| 摘 要： | The Na-K-2Cl co-transporter(NKCC)is anintegral membrane protein responsible for coupledelectroneutral transport of1Na:1K:2Cl across theplasma membrane in a variety of ani mal cells.There are two functionally distinct isoforms ofNKCC.The apically expressed adsorptiveisoforms(NKCC2)has been found only in the kidney,whereas the basolaterally located secretoryisoform(NKCC1)is expressedin several different tissues,where it participatesin vectorial salt transport,iongradient homeostasis,and …
|
| 关 键 词： | 耳蜗 形态学 免疫组织化学 基因传输 |
| 收稿时间： | 2006-01-29 |
Localization of NKCC1 in the cochlea and morphology of the cochlea in NKCC1-knockout mice |
| |
| Chu Hanqi,Xiong Hao,Zhou Xiaoqin,Huang Xiaowen,Zhou Liangqiang,Cui Yonghua.Localization of NKCC1 in the Cochlea and Morphology of the Cochlea in NKCC1-Knockout Mice[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(3):374-377. |
| |
| Authors: | Chu Hanqi Xiong Hao Zhou Xiaoqin Huang Xiaowen Zhou Liangqiang Cui Yonghua |
| |
| Institution: | 1. Department of Otolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Department of Respiratory Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
| |
| Abstract: | The distribution of the Na-K-2Cl co-transporter (NKCC1) in the cochlear K cycling pathway in cochlea and cochlear histological changes in the NKCC1 knockout mice were investigated. By using immunohistochemistry and toluidine blue staining, the localization of NKCC1 in cochlea of the C57BL/6J mice and the cochlear histological changes in the NKCC1 knockout mice were observed. It was found that the NKCC1 was expressed mainly in the stria marginal cells and the fibrocytes in the inferior portion of the spiral ligament in the adult C57BL/6J mice. Subpopulation of the fibrocytes in the suprastrial region and the limbus was also moderately immunoreactive. While in the cochlea of the NKCC1 knockout mice, Reissner's membrane was collapsed and scala media disappeared, accompanied with the loss of inner hair cells, outer hair cells and the support cells. The tunnel of Corti was often absent. All the findings suggested the localization of NKCC1 in the cochlea was closely correlated with cochlear K cycling. Loss of NKCC1 led to the destruction of the cochlear structures, and subsequently influenced the physiological function of cochlea. |
| |
| Keywords: | Na-K-2Cl co-transporter gene knockout K cycling Immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
