宫内窘迫胎鼠脑及肝组织自由基损伤的研究

目的 探讨宫内窘迫胎鼠自由基损伤情况及对不同脏器的影响。方法 通过钳夹双侧子宫动、静脉造成宫内胎鼠完全缺血缺氧,其中Ａ组钳夹５分钟（胎鼠１３只）;Ｂ组钳夹１０分钟（胎鼠１０只）;Ｃ组钳夹２０分钟（胎鼠１１只）;对照组为假手术组（胎鼠１３只）。另一部分钳夹后再灌注,其中Ｄ组钳夹１０分钟后再灌注５分钟（胎鼠１１只）,Ｅ组钳夹１０分钟后灌注２０分钟（胎鼠８只）;以Ｂ组为对照。以比色法测定脑、肝组织匀浆后

Changes of free radicals in fetal rats brain and liver following intrauterine fetal distress

Objective To analysis the effect of superoxide dismutase (SOD) and malondialdehyde (MDA) on different organs in intrauterine fetal distress. Methods The times of clamping uterine artery in A, B, C groups were 5, 10, 20 minites respectively. By clamping the matenal uterine vessels, we made the model of fetal rat distress. Slam group (13 pups) was used as control. After clamping 10 minutes, Group D (11 pups) was reperfused 5 minutes, and E (8 pups) 20 minutes. We measured the level of SOD and MDA in fetal brains and livers. Results The activity of cerebral SOD in group B and C decreased significantly than that of control ( P < 0.05, P <0.01). MDA in group C increased significantly than that of control ( P <0.05). As for liver, only SOD in group C decreased significanlty ( P <0.05). In that of reperfusion group, only in group E, cerebral SOD and MDA changed significantly ( P <0.01, <0.05), livers SOD decreased significanlty ( P <0.05).Conclusion After severe intrauterine fetal distress, changes of free radicals occurred in brains firstly, which provides the proof of early treatment for neonatal hypoxia ischemia brain damage.