胃肠乐对幽门结扎胃溃疡胃黏膜EGF和EGFR的影响

目的]探讨胃肠乐抗溃疡的机制及对胃黏膜细胞的保护作用。方法]将48只大鼠随机分为正常对照组、模型对照组、胃肠乐高剂量组、胃肠乐中剂量组、胃肠乐低剂量组、洛赛克组;用游标尺计算各组溃疡指数,采用免疫组织化学分析方法检测胃黏膜表皮生长因子(EGF)、表皮生长因子受体(EGFR)的表达及图像分析。结果]胃肠乐高、中剂量组胃黏膜全层EGF、EGFR表达明显(其积分光密度值、平均灰度及阳性细胞占总面积的百分比与模型对照组相比有显著差异)(P<0.05)。结论]胃肠乐抗溃疡,促进溃疡愈合的作用机制可能与其通过增加EGF、EGFR表达而起到胃黏膜细胞保护作用有关。

Effect of Weichangle on EGF and EGFR in rats with gastric ulcer induced by pyloric ligation

Objective] To investigate the mechanism of anti-ulcer function and protective effect to gastric mucosa cells of Weichangle(WCL). Methods] Fourty-eight SD rats were divided randomly into 5 groups: normal group, model group, WCL high-dosage group, WCL middle-dosage group, WCL low-dosage group and Omeptazole group. The index of ulcer(UI) was measured by a vernier, the expression of epidermal growth factor (EGF) and its receptor (EGFR) were detected by immunohistochemistry (SABC), and the microimagine was analysed by MPISA. Result] The expression of EGF and EGFR in high and mild dosage group of WCL were obviously higher than those of the model group (P<0.05). Conclusion] WCL has anti -ulcer effect and make the ulcer healed. The mechanisms maybe relate to its effect of promoting the expression of EGF and EGFR, and thereby protecting the gastric mucosa.