Neuropeptide F-immunoreactivity in the tetrathyridium ofMesocestoides corti (Cestoda: Cyclophyllidea) |
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| Authors: | Gabriela Hrckova David W. Halton Aaron G. Maule Gerry P. Brennan Chris Shaw Colin F. Johnston |
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| Affiliation: | (1) Parasitological Institute, Slovak Academy of Sciences, 040 01 Kosice, Slovakia;(2) Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, The Queen's University of Belfast, BT7 1NN Belfast, UK;(3) School of Clinical Medicine, The Queen's University of Belfast, BT7 1NN Belfast, UK |
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| Abstract: | The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage ofMesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30–39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified. |
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