| 人PFP、GrB共表达诱导喉癌Hep-2细胞凋亡的研究 |
| |
| 引用本文： | 李秀英,夏良平,赖延东. 人PFP、GrB共表达诱导喉癌Hep-2细胞凋亡的研究[J]. 免疫学杂志, 2009, 25(2) |
| |
| 作者姓名： | 李秀英 夏良平 赖延东 |
| |
| 作者单位： | 李秀英,LI Xiuying(暨南大学药学院基因组药物研究所);夏良平,XIA Liangping(中山大学肿瘤防治中心华南肿瘤学国家重点实验室);赖延东,LAI Yandong(广州医学院化学致癌研究所,广州,510182) |
| |
| 基金项目： | 广东省医学科研课题，广东省自然科学基金 |
| |
| 摘 要： | 目的 探讨人穿孔素(PFP)、颗粒酶B(GrB)共表达是否可以诱导人的喉癌细胞系Hep-2的凋亡及其作用的机理.方法 利用脂质体2000将PFP、GrB共表达载体pVAX1-PIG(即pVAX1-PFP-IRES-GrB)转染人喉癌Hep-2细胞,采用荧光染料Hoechst33342法、流式细胞仪(FCM)检测、透射电镜观察Hep-2细胞的凋亡情况.以激光共聚焦显微镜检测重组载体转染后的Hep-2细胞内[Ca2+];浓度的变化,并探讨其作用机理.结果 pVAX1-PIG转染组的Hep-2细胞大量凋亡且其凋亡率显著高于对照组(P＜0.05),透射电镜研究显示单个Hep-2细胞内亦出现凋亡的特征.Hep-2细胞内[Ca2+];的浓度发生了变化,且由FI值增大可知细胞胞浆内[Ca2+];的浓度升高.结论 PFP、GrB共表达能够诱导人Hep-2细胞的凋亡,且凋亡的发生与细胞胞浆内[Ca2+];的浓度升高有关.
|
| 关 键 词： | 凋亡 激光共聚焦显微镜技术 透射电镜 流式细胞仪分析 |
Apoptosis of human Hep-2 cells induced by pore-forming protein and granzyme B |
| |
| LI Xiuying,XIA Liangping,LAI Y,ong College of Pharmacy,Jinan University,Guangzhou ,China. Apoptosis of human Hep-2 cells induced by pore-forming protein and granzyme B[J]. Immunological Journal, 2009, 25(2) |
| |
| Authors: | LI Xiuying XIA Liangping LAI Y ong College of Pharmacy Jinan University Guangzhou China |
| |
| Affiliation: | LI Xiuying,XIA Liangping,LAI Y,ong College of Pharmacy,Jinan University,Guangzhou 510632,China |
| |
| Abstract: | Objective To investigate the effect of pore-forming protein(PFP)and granzyme B(GrB)on apoptosis of human Hep-2 cells and the mechanisms.Methods The eukaryotic co-expression recombinant pVAX1-PIG was transfected into Hep-2 cells.The apoptosis of Hep-2 cells were identified by the assays of electronic microscopy,flow cytometry,and Hoechst33342 staining.The intracellular Ca2+i concentration in Hep-2 cells was detected by the assays of calcium fluorescent probe Fluo-3/AM and laser confocal microscopy.Results He... |
| |
| Keywords: | apoptosis laser confocal microscopy electronic microscope flow cytometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
