双功能基因APE1对多发性骨髓瘤疗效的影响

目的探讨MM细胞APE1基因表达及定位对MM疗效的影响。方法采用双标免疫荧光结合激光共聚焦显微镜和免疫细胞化学分析MM细胞系KM3,并在32例MM患者和10例正常自愿者骨髓标本中检测APE1蛋白表达及定位。结果MM患者骨髓标本有APE1和CD38蛋白共表达;在作为阳性对照的MM细胞系KM3中胞核、胞核/胞浆、胞浆均有APE1表达,其中尤以胞核明显;APE1胞浆阳性分度在正常对照组、初治组、复发或难治组间依次增高(P<0.01或0.05)。结论APE1蛋白表达强度与MM疗效有关,APE1基因表达增强可能是MM患者预后不良的指标之一。

Effects of bifunctional gene APE1 on the therapeutic effect of multiple myeloma

Objective To investigate the effects of the expression and location of apurinic/apyrimidinic endonuclease (APE1) protein on the therapeutic effect of multiple myeloma (MM). Methods Forty-two bone marrow specimens were collected from 32 MM patients and 10 normal volunteers. Expression and location of APE1 protein in the MM cell line KM3 and the 42 specimens were detected by immunofluorescence double staining, cofocal laser scanning microscopy, and immunocytochemical staining. Results APE1 and CD38 were co-expressed in bone marrow specimen of MM. Nuclear, nucleus/cytoplasmic, and cytoplasmic staining of APE1 protein were found in MM. Expression of APE1 gene in nucleus were significantly elevated compared to that in nucleus/cytoplasm and cytoplasm. In normal control group, untreated patients group, and relapse/refractory group, the positive degree of cytoplasmic staining were increased in turn ( P < 0.01 or 0.05). Conclusion Expression degree of APE1 protein is associated with therapeutic effect of MM, which suggest that high expression of APE1 protein may predict poor prognosis.