| 胶质细胞源性神经营养因子体外对脊髓运动神经元的作用 |
| |
| 引用本文： | 宋学琴,吴淑玉,李春岩,王丽琴,王晓娟. 胶质细胞源性神经营养因子体外对脊髓运动神经元的作用[J]. 细胞与分子免疫学杂志, 2003, 19(5): 490-492 |
| |
| 作者姓名： | 宋学琴 吴淑玉 李春岩 王丽琴 王晓娟 |
| |
| 作者单位： | 河北医科大学第二医院神经内科,河北,石家庄,050000 |
| |
| 摘 要： | 目的 :观察不同浓度的胶质细胞源性神经营养因子 (GDNF) ,对大鼠胚胎脊髓运动神经元生长活性的作用。方法 :取大鼠胚胎脊髓腹侧组织体外分离 ,进行原代细胞培养 ,应用抗神经微丝单克隆抗体 (mAb)SMI32进行运动神经元的免疫细胞化学染色 ,从细胞形态学及应用MTT比色法 ,研究GDNF对大鼠脊髓运动神经元的影响。结果 :GDNF能明显促进体外培养的大鼠脊髓运动神经元存活及突起的生长 (P <0 .0 5 ) ,且具有剂量依赖的趋势。结论 :不同浓度的GDNF对体外培养的大鼠胚胎脊髓运动神经元 ,有不同程度的促生长作用
|
| 关 键 词： | 胶质细胞源性神经营养因子 脊髓运动神经元 体外培养 抗神经微丝单克隆抗体 |
| 文章编号： | 1007-8738(2003)05-490-03 |
| 修稿时间： | 2002-10-04 |
Effect of glia cell line-derived neurotrophic factor on cultured spinal motor neurons from embryonic rat |
| |
| SONG Xue qin,WU Shu yu,LI Chun yan,WANG Li qin,WANG Xiao juan Neurology Department,Second Hospital of Hebei Medical University,Shijiazhuang ,China. Effect of glia cell line-derived neurotrophic factor on cultured spinal motor neurons from embryonic rat[J]. Chinese journal of cellular and molecular immunology, 2003, 19(5): 490-492 |
| |
| Authors: | SONG Xue qin WU Shu yu LI Chun yan WANG Li qin WANG Xiao juan Neurology Department Second Hospital of Hebei Medical University Shijiazhuang China |
| |
| Affiliation: | Department of Neurology,Second Hospital of Hebei Medical University, Shijiazhuang 050000, China. |
| |
| Abstract: | AIM: To observe the effects of various concentrations of glia cell line derived neurotrophic factor (GDNF) on the growth of cultured spinal motor neurons from embryonic rat. METHODS: Neurons were isolated from embryonic rat spinal ventral tissue and then were primarily cultured in vitro . Morphological feature of neurons was examined by immunocytochemical staining with mAb SMI 32. The effect of GDNF on growth of spinal motor neurons was detected by MTT colorimetry. RESULTS: GDNF could significantly enhance the survival and neurite outgrowth of rat spinal motor neurons cultured in vitro in dose dependent tendency. CONCLUSION: Varying concentrations of GDNF can enhance the growth of embryonic rat spinal motor neurons to various extent. |
| |
| Keywords: | glia cell line derived neurotrophic factor spinal motor neuron culture mAb SMI32 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
