Comparative analysis of c‐erbB‐2 (HER‐2/neu) in squamous cell carcinoma of the tongue: does over‐expression exist? And what is its correlation with traditional diagnostic parameters?

Objectives:  Over-expression of the proto-oncogene c-erbB-2 (HER-2/ neu ) has been shown to be a prognostic marker in many kinds of cancer, whereas conflicting data exist about the prevalence of HER-2/ neu over-expression in squamous cell carcinoma (SCC) of the tongue. The status of Her-2/ neu was evaluated in a series of SCC of the tongue to verify the frequency of over-expression of HER-2/neu and evaluate the correlation with traditional diagnostic parameters of this neoplasm.
Method:  Fourty patients with SCC of the tongue were investigated for over-expression of the protein through immunohistochemistry using CB11 antibody, the Hercep Test^® kit and FISH.
Results:  Data obtained using the Hercep Test^® differ from published reports concerning the over-expression of Her-2/ neu and there was no correlation between levels of expression of Her-2/ neu and other clinico-pathological and/or prognostic parameters. Of the 40 specimens, using CB11 we obtained results in line with published reports; however, with the Hercep Test^® we found only 1 positive case (2.5%) (score 3+).
Conclusion:  These data, confirmed by FISH, suggest that Her-2/ neu is not a suitable marker that could play a primary role in the clinical-therapeutic management of SCC of the tongue.