Failure to prolong pancreatic islet allograft survival in rats with donor-specific blood transfusions and immunosuppression

Abstract. The effects on pancreatic islet allograft survival of donor-specific blood transfusions (DST) in combination with pre-and posttransplant immunosuppression were studied. A total of 12 groups of rats ( n = 105) with chemically induced diabetes underwent islet allotransplantation. Multiple DST or third-party blood transfusions (TPT) were given prior to transplantation. Pretransplant immunosuppression consisted of azathioprine and prednisolone, and low-dose cyclosporin A was used for posttransplant immunosuppression. TPT, as well as separate or combined pre-and posttransplant immunosuppression without blood transfusions, did not prolong islet allograft survival. DST resulted in either primary nonfunction of the islet allografts or a markedly decreased islet allograft survival. These findings contrast with the beneficial effect of DST on whole-organ allograft survival in rats previously described by others.