林蛙油对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响

目的:研究林蛙油对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响。方法:以大强度耐力训练大鼠为模型,55只42 d龄雄性Wistar大鼠为对象,随机分为5组:静止对照组(C组)、运动对照组(M组)、运动+低剂量林蛙油组(OML组),运动+中剂量林蛙油组(OMM组),运动+高剂量林蛙油组(OMH组),每组10只(剔除不符合实验要求的大鼠5只)。每天灌胃(ig)1次,林蛙油组剂量分为0.5,1.0,3.0 g·kg-1,灌胃体积为5 mL·kg-1,C,M组ig等量生理盐水。42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮等生化指标。结果:体重:运动对照组小于静止对照组(P<0.05);林蛙油各组大于运动对照组(P<0.05),组间无显著差异。力竭游泳时间:运动对照组与静止对照组无明显差异;林蛙油各组明显长于运动对照组(P<0.01),且随剂量增大而延长。血清睾酮水平:运动对照组(P<0.01)低于静止对照组;林蛙油各组分别为(4.96±1.60),(5.19±1.60),(5.35±1.66)nmol·L-1,高于运动对照组(P<0.01),组间无显著差异。血清皮质酮水平:各组间均无显著差异。血清睾酮与皮质酮比值变化与睾酮变化较为一致。肝、肌糖原水平:运动对照组(P<0.01)低于静止对照组;林蛙油各组高于运动对照组(分别为P<0.05,P<0.01),组间无显著差异。促黄体生成素、促卵泡激素各组间无显著差异。血尿素水平:运动对照组(P<0.01)高于安静对照组;林蛙油各组低于运动对照组(P<0.01),且组间无显著差异。血红蛋白水平:运动对照组(P<0.01)低于静止对照组;林蛙油各组高于运动对照组(P<0.01),且组间无显著差异。结论:林蛙油可以减轻血睾酮受高强度运动量的影响,并维持在正常生理水平;可以促进蛋白质合成,抑制氨基酸和蛋白质分解,提高运动训练大鼠血红蛋白含量和糖原的储备。

Effect of Oviductus Ranae on Testosterone Content, Substance Metabolism and Exercise Capacity in Rats Receiving Exercise Training

Objective: To study the effects of Oviductus Ranae on the content of testosterone, substance metabolism and anti-fatigue ability of rats after exercise. Method: By using the model of high-intensity endurance training, fifty-five male Wistar rats were randomly divided into 5 groups, with 10 in each group. The rats which did not meet the requirement were removed:still in control group (C group), motion control group (M group), exercise +ig low-dose Oviductus Ranae group (OML group), exercise+ig middle-dose Oviductus Ranae group (OMM group), and exercise+ig high-dose Oviductus Ranae group (OMH group). Gavage was performed using professional device once a day. The rats in Oviductus Ranae groups were gavaged with 0.5, 1.0, 3.0 g·kg^-1 with ig volume of 5 mL·kg^-1. The rats in C and M groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: The body weights of the rats in M groups were lower than those in C group (P<0.05), and those in all doses of Oviductus Ranae groups were higher than in M group (P<0.05) which showed no differences between groups. Swimming time in all doses of Oviductus Ranae groups was longer than M group (P<0.01)with a dose-effect relationship, But there were no differences between C and M groups. Serum testosterone in M group was lower than C group (P<0.01), in the same time, serum testosterone was higher in all doses of Oviductus Ranae groups than M group [OML:(4.96±1.60) nmol·L^-1;OMM:(5.19±1.60) nmol·L^-1;OMH:(5.35±1.66) nmol·L^-1, P<0.01]. The serum corticosterone levels in each group showed no significant differences. Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. Liver glycogen and muscle glycogen (P<0.01) in M group were lower than in C group, and in all doses of Oviductus Ranae groups were higher than in M group (liver glycogen, P<0.05;muscle glycogen, P<0.01). There were no differences in luteinizing hormone and follicle-stimulating hormone between groups. The serum urea nitrogen in M group was higher than C group (P<0.01), and in all doses of Oviductus Ranae groups was lower than the M group (P<0.01). Hemoglobin in M group was lower than C group (P<0.01), and in all doses of Oviductus Ranae groups were higher than in M group(P<0.01). Conclusion: Oviductus Ranae can alleviate the impact of high-intensity exercise on serum testosterone, and maintained it at normal physiological levels;it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.