正交试验优选复方广金钱草颗粒的提取工艺

目的:优选复方广金钱草颗粒的提取工艺。方法:以夏佛塔苷、总黄酮提取量和干膏得率的综合评分为指标,通过正交试验考察加水量、提取时间及温度对复方广金钱草颗粒水提工艺的影响;以总多糖、总黄酮含量及除杂量的综合评分为指标,通过正交试验优选水提液的醇沉工艺;以干膏得率、总黄酮及23-乙酰泽泻醇B含量的综合得分为指标,通过正交试验优选复方广金钱草颗粒的醇提工艺。采用UPLC测定夏佛塔苷及23-乙酰泽泻醇B含量,流动相分别为甲醇-0.4%磷酸溶液(32∶68)和乙腈-水(73∶27);采用UV测定总黄酮和总多糖含量。结果:最佳水提工艺条件为加8倍量水回流提取3次,每次1.5 h;最佳醇沉工艺为醇沉浓度60%,初始密度1.0 g·mL-1,醇沉时间8 h;最佳醇提工艺为加8,6倍量60%乙醇提取2次,提取时间分别为1,0.5 h。结论:优选的提取工艺稳定可行,适用于复方广金钱草颗粒的工业生产。

Optimization of Extraction Technology for Fufang Guangjinqiancao Granules by Orthogonal Design

Objective: To optimize extraction technology of Fufang Guangjinqiancao granules. Method: With composite score of contents of schaftoside and total flavonoids, yield of dry extract as index, orthogonal test was adopted to optimize water extraction technology by taking the amount of water, extracting time and temperature as factors;taking composite score of contents of total polysaccharides and total flavonoids, removal rate of impurity as index, alcohol precipitation process of water extract was optimized by orthogonal test.With composite score of contents of 23-acetyl alisol B and total flavonoids, yield of dry extract as index, alcohol extraction technology of this compound was optimized by orthogonal test.Contents of schaftoside and 23-acetyl alisol B were determined by UPLC, mobile phase were methanol-0.4% phosphoric acid solution(32:68) and acetonitrile-water(73:27), respectively;UV was employed to measure contents of total flavonoids and total polysaccharides. Result: Optimum water extraction conditions were as follows:extracted 3 times with 8-folds of water for 1.5 h each time;optimal alcohol precipitation process was:alcohol precipitation concentration 60%, initial density of water extract 1.0 g·mL^-1, alcohol sedimentation time for 8 h;optimum alcohol extraction conditions were as follows:extracted 2 times with 8, 6-folds of 60% ethanol, extracting time of 1.0, 0.5 h, respectively. Conclusion: These optimized extraction processes are stable, feasible and suitable for industrial production of Fufang Guangjinqiancao granules.