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| Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响 | |
| 引用本文： | 尚超,李巍,郭艳,富伟能,孙开来.Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响[J].山东医药,2011,51(11):1-3. |
| 作者姓名： | 尚超 李巍 郭艳 富伟能 孙开来 |
| 作者单位： | 1. 中国医科大学,沈阳,110001 2. 中国医科大学附属第一医院 3. 中国医科大学附属口腔医院 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的探讨Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响及机制。方法以Hep2细胞为对照组,转染pcDNA3.1-Mst1的Hep2细胞为转染组,SB203580处理的Mst1转染细胞为处理组。采用MTT法测定三组细胞的增殖抑制率,双标流式细胞法检测其凋亡率,用Western blot法检测三组细胞中Mst1蛋白、磷酸化p38（p-p38）和总p38蛋白（t-p38）的表达情况。结果与对照组相比,转染组Mst1和p-p38蛋白表达增强明显t,-p38的表达无明显变化,凋亡率由2.46%升高至7.37%,细胞增殖抑制率为41.8%。与转染组相比,处理组p-p38蛋白表达增强降低t,-p38表达无明显变化,细胞凋亡率由7.37%降低至2.65%,细胞抑制率为-10.1%,细胞增殖活力显著降低。结论 Mst1基因能够通过p38 MAPK通路参与喉癌细胞凋亡和增殖的调控。 |
| 关 键 词： | Mst1基因 喉肿瘤 喉癌 细胞凋亡 |
Effects of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells |
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| SHANG Chao,LI Wei,GUO Yan,FU Wei-neng,SUN Kai-lai.Effects of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells[J].Shandong Medical Journal,2011,51(11):1-3. | |
| Authors: | SHANG Chao LI Wei GUO Yan FU Wei-neng SUN Kai-lai |
| Institution: | SHANG Chao1,LI Wei,GUO Yan,FU Wei-neng,SUN Kai-lai(1 China Medical University,Shenyang 110001,P.R.China) |
| Abstract: | Objective To investigate the influence and mechanism of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells.Methods The Hep2 cells were regarded as control group,and the Hep2 cells transfected with pcDNA3.1-Mst1 as Mst1 transfected group,the Mst1 transfected group cells treated with SB203580 as SB203580 treated group.In the three groups,the inhibitory rate of cell growth was detected by MTT,the apoptosis was detected by flow cytometry,and the protein expression of Mst1 and phospho... |
| Keywords: | Mst1 gene laryngeal tumor laryngeal carcinoma apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |