| HLA-B对喉癌Hep2细胞生物学行为的影响 |
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| 引用本文： | 郭艳,富伟能,尚超,钟鸣,孙开来. HLA-B对喉癌Hep2细胞生物学行为的影响[J]. 山东医药, 2011, 51(11): 21-22,33 |
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| 作者姓名： | 郭艳 富伟能 尚超 钟鸣 孙开来 |
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| 作者单位： | 1. 中国医科大学口腔医学院,沈阳,110007
2. 中国医科大学基础医学院 |
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| 基金项目： | 辽宁省自然科学基金资助项目 |
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| 摘 要： | 目的探讨人类白细胞组织相容抗原（HLA）-B在喉癌发生、发展中的作用。方法采用RNA干涉技术下调Hep2细胞中HLA-B基因表达。用干涉效应最明显、转染siRNA的细胞作为观察组,转染PBS及无义siRNA的细胞作为对照1组及对照2组。采用流式细胞仪、MTT和细胞体外侵袭力分析方法检测各组细胞凋亡率、增殖情况、细胞周期及局部侵袭能力。结果 RNA干涉7 d观察组细胞凋亡率明显高于两对照组,细胞周期阻滞于S期;细胞增殖能力及局部侵袭能力明显强于两对照组,P均〈0.05。结论 HLA-B表达降低或缺失在喉癌的发生、发展中起促进作用。
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| 关 键 词： | 喉肿瘤,喉癌 人类白细胞组织相容抗原-B RNA干涉 生物学行为 |
Effect of HLA-B on the biological behavior of laryngeal carcinoma Hep2 cell |
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| GUO Yan,FU Wei-neng,SHANG Chao,ZHONG Ming,SUN Kai-lai. Effect of HLA-B on the biological behavior of laryngeal carcinoma Hep2 cell[J]. Shandong Medical Journal, 2011, 51(11): 21-22,33 |
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| Authors: | GUO Yan FU Wei-neng SHANG Chao ZHONG Ming SUN Kai-lai |
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| Affiliation: | GUO Yan1,FU Wei-neng,SHANG Chao,ZHONG Ming,SUN Kai-lai(1 China Medical University,School of Scomatology,Shenyang 110007,P.R.China) |
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| Abstract: | Objective To investigate the effect of HLA-B on the occurrence and development of laryngeal carcinoma.Methods RNA interference technology was used to down-regulate the expression of human leukocyte antigen(HLA)-B in Hep2 cells.Cells transfected with siRNA at most effective interference were used as observed group,while cells with PBS and nonsense siRNA transfection as control group 1 and 2.Then flow cytometry,MTT assay and cell invasion in vitro were used to detect the biological effect of Hep2 cells induce... |
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| Keywords: | laryngeal tumor laryngeal carcinoma human leukocyte antigen-B RNA interference biological behavior |
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