异种去细胞真皮基质在体内转归的初步研究

目的 初步研究异种（猪）去细胞真皮基质在体内的转归。方法 经曲通（Triton）X-100、胰蛋白酶处理得到异种（猪）去细胞真皮基质后，将其包埋于SD大鼠皮下，于术后1、2、4、6、8、12、16、20和30周观察异种（猪）去细胞真皮基质的大体形态及组织学变化。结果 制备的异种（猪）去细胞真皮基质去细胞成分，胶原纤维排列有序，基底膜结构完整。大体观察异种（猪）去细胞真皮基质原始形态随时间延长而逐渐模糊．组织学观察异种（猪）去细胞真皮基质1周以炎性细胞浸润为主，以后成纤维细胞、毛细血管逐渐增多，真皮胶原排列逐渐规则、致密。结论 异种（猪）去细胞真皮基质可在体内作为支架长期存在，并可诱导自身成纤维细胞及毛细血管有序长入。

Preliminary study on outcome of xenogeneic (porcine) acellular dermal matrix in vivo

Objective To investigate the outcome of xenogeneic (porcine) acellular dermal matrix in vivo. Methods Xenogeneic (porcine) acellular dermal matrix was prepared by removing cellular components with trypsin and triton X100 and was embedded under the skin of SD rat. Gross and histological changes were observed periodically. Results There was no cellular components but with intact basal membrane, and collagen was found to have a regular arrangement in xenogeneic (porcine) acellular dermal matrix. Grossly, the xenogeneic (porcine) acellular dermal matrix was found to disintegrate gradually. Histological examination revealed that xenogeneic (porcine) acellular dermal matrix was mainly infiltrated by inflammatory cells in one week after implantation. After one week, fibroblasts and capillary vessel increased in number gradually, and collagen began to arrange densely in regular manner. Conclusion Xenogeneic (porcine) acellular dermal matrix disintegrates gradually after transplantation, inducing growth of fibroblasts and capillaries.