高效液相色谱法测定血浆中儿茶酚胺

目的：建立用高效液相色谱（HPLC）电化学检测器同时测定血浆中儿茶酚胺水平的方法，以诊断哮铬细胞瘤和神经母细胞瘤。方法：利用离子对提取血浆中儿茶酚胺，电化学检测器检测血浆中儿茶酚胺水平，并测定其回收率，线性范围，精密度及最低检查限度，参考18例正常人儿茶酚胺水平建立正常参考范围。结果：肾上腺素，去甲肾上腺素回收率分别为86．2％和90．5％，该方法测定肾上腺素在0．2－11．6nmol.L^-1内呈良好线性关系，最低检查限度为0．05nmol.L^-1，批内，批间变异系数别小于10．7％和11．1％。结论：本法肜于检测血浆中儿茶酚胺水平具有准确，灵敏，简便的特点，适合临床诊断。

Simultaneous determination of catecholamine levels in plasma by high performance liquid chromatography]

OBJECTIVE: To use a high performance liquid chromatography with electrochemical detection to simultaneously determine catecholamine(CA) levels in plasma and to diagnose chromaffin cell tumors and neuroblastoma. METHODS: The plasma samples after flowing extraction by ion-moderated partition were determined with electrochemical method to detect CA levels in plasma; the lowest detective limit, the precision, recovery, sensitivity of CA levels were tested and a reference range was established based on the respective data of 18 healthy persons. RESULTS: The recovery rates of epinephrine and norepinephrine were 86.2% and 90.5%. The method of testing epinephrine was linear at the range of 0.2 to 10.6 nmol.L-1 with 0.05 nmol.L-1 of the lowest detective limit. The intra- and inter-assay coefficients of variation were lower than 10.7% and 11.1%, respectively. CONCLUSION: The method is simple, accurate, sensible in the clinical diagnosis of CA levels.