Neuro-2a细胞替代神经元原代培养进行神经轴突测量实验研究

目的 建立使用小鼠神经母细胞瘤Neuro-2a细胞替代原代培养神经元进行神经轴突测量的方法,用于神经损伤研究.方法 使用浓度200 μmol/L、500 μmol/L、1 000μmol/L过氧化氢处理Neuro-2a细胞12h,戊二醛固定,生物染色,在光学显微镜下手动测量突起长度.结果 过氧化氢可剂量依赖性引起神经突起逐渐回缩变短,1 000 μmol/L过氧化氢处理组光镜下见不到明显突起,突起测量数据显示,过氧化氢处理后突起长度与未经处理细胞有显著差异.结论 Neuro-2a细胞在一定程度上可替代原代培养神经元进行轴突测量研究,该测量方法可用于神经轴突再生研究.

Neuro-2a cells as a substitute for primary neuron culture in neurite measurement

Objective To investigate the utilization of mouse neuroblastoma Neuro-2a cells as a substitute for primary neuron culture in neurite measurement and its application in neuronal injury in vitro.Methods Hydrogen peroxide(H2O2) at 200 μmol/L,500 μmol/L and 1 000 μmol/L were administered to Neuro-2a cells for 12 hours.Cells were fixed with glutaraldehyde and stained for the length measurement of neurites manually under the light microscope.Results Administration of hydrogen peroxide can dose dependently result in the shringkage of neurites,and neurites were unmeasurable in cells treated with H2O2 at 1 000 μmol/L.Our data demonstrated a significant difference in neurite lengths between H2O2 treated cells and those untreated.Conclusion Neuro-2a cells can be used as a partial substitute for primary neuron culture in neurite measurement and applied in vitro neuronal injury study.