肠营养素对小肠粘膜上皮细胞分化的影响

目的：探讨表皮生长因子（ＥＧＦ）、碱性成纤维细胞生长因子（ｂＦＧＦ）、胰岛素样生长因子－１（ＩＧＦ－１）、Ｌ－谷氨酰胺（Ｇｌｎ）、精氨酸、重组人生长激素（ｒｈＧＨ）和胰岛素对原代培养的小肠粘膜上皮细胞分化的影响。 方法：孕龄１７￣１９天Ｗｉｓｔａｒ系孕鼠胎仔小肠制备成小肠绒毛细胞团悬液，培养４８ｈ后加入上述肠营养素，通过测定ＡＬＰ活性观察不同剂量肠营养素对贴壁生长的肠上皮细胞分化的作用。 结果：当

Direct influence of intestinal nutrients over the differentiation of mucosal epithelium of small intestine in primary culture

Objectives:To investigate the effects of EGF,bFGF,IGF 1,Gln,arginine,rhGH,and insulin on the differentiation of small intestinal epithelium in the primary culture. Methods:The epithelial organoids of small intestine were obtained from wistar fetal rats,and were successfully cultuerd.After 48h incubation,each group received one kind of intestinal nutrients at different levels alone.Alkaline phosphatase(ALP) activity of epithelial cells was measured to identify the differentiation of small intestinal epithelium. Results:While it had trophic effect,the intestinal nutrient could exert direct influence over the epithelial differentiation of small intestine.EFG,IGF 1,Gln,insulin,and rhGH accelerated cell differentiation,but arginine and bFGF decreased epithelial differentiation. Conclusions:Besides their trophic effects,the ability of intestinal nutrients to induce the differentiation of intestinal epithelial cells must be paid more attention.