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Transformation of Penicillium nalgiovense with the amdS gene of Aspergillus nidulans
Authors:Rolf Geisen  Lothar Leistner
Affiliation:(1) Bundesanstalt für Fleischforschung, Institut für Mikrobiologie, Toxikologie und Histologie, D-8650 Kulmbach, Germany
Abstract:Summary Penicillium nalgiovense was transformed with the amdS gene from Aspergillus nidulans as a selectable marker. The vector apparently integrated at multiple sites into the chromosomes of the transformants, which were mitotically stable. A transformation efficiency of 12 transformants/mgrg vector DNA was achieved when the expression phase was prolonged to 8 h.
Keywords:Penicillium nalgiovense  Transformation  amdS gene
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