| 高效液相色谱法测定上感清口服液中黄芩苷的含量 |
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| 引用本文： | 张国添,张敏怡. 高效液相色谱法测定上感清口服液中黄芩苷的含量[J]. 中国实用医药, 2009, 4(30): 48-49 |
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| 作者姓名： | 张国添 张敏怡 |
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| 作者单位： | 广州市番禺区人民医院/中山大学附属第一医院药剂科,514000 |
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| 摘 要： | 目的采用反相高效液相色谱法测定上感清口服液中黄芩苷的含量。方法以甲醇-水-磷酸（47：53：0.2）为流动相,Lichrospher R100（4.6mm×250mm,5μm）为固定相,流速为1.0ml/min,检测波长为280nm。结果黄芩苷在0.24～1.20μg范围内有良好的线性关系,回收率为98.24%,RSD为1.20%（n=6）。结论方法简单、灵敏度高,可用于上感清口服液中黄芩苷的含量测定。
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| 关 键 词： | 上感清口服液 黄芩苷 HPLC |
Determination of Baicalin in Shangganqing Oral Liquid by HPLC |
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| Affiliation: | ZHANG Guo-tia( Panyu District People＇ s Hospital, Gnangzhou, Guangdong 514000, China) |
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| Abstract: | Objective To develop RP-HPLC method for the assay of Baicalin in Shangganqing Oral Liquid.Methods The mobile phase consist of methanol-water-Phosphate（47：53：0.2）.Separation was performed on a Lichrospher R 100（4.6 mm×250 mm,5 μm）column.The flow rate was 1.0 ml/min.The UV detection was set at 280 nm.Results Baicalin had a good linear in the range of 0.24-1.20 μg.The average recovery of Chlorogenic acid was 98.24%with RSD 1.20%（n=6）.Conclusion The method was simple,sensitive and rapid and it can be applied to Baicalin content determination of Shangganqing Oral Liquid. |
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| Keywords: | HPLC |
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