胃癌8号染色体等位基因杂合性缺失及其临床相关性研究

近年来，人们认为染色体上等位基因缺失或失活是肿瘤形成和演进的一个重要机制。应用从人类染色体上分离出的克隆──Ｄ８Ｓ７作为探针，采用ＳｏｕｔｈｅｒｎＢｌｏｔ方法检测了２０例胃癌病员的癌组织及其相应的癌旁组织基因组ＤＮＡ８号染色体等位基因杂合性丢失（ＬＯＨ），结果发现，２５％（５／２０）的胃癌组织以及１５％（３／２０）的癌旁组织中存在染色体８Ｐ上等位基因缺失或丢失，早期和晚期胃癌均存在这种分子遗传学改变，提示人类８号染色体短臂上存在肿瘤抑制基因，此基因正常等位基因缺失或丢失参与胃粘膜细胞癌变及其胃癌的演化和进程。

A Study on the Clinical Significance of the Loci LOH on Chromosomal 8 in Human Gastric Carcinomas

Recently,loss or inactivation of specific chromosomal loci has been considered to be one of the importment mechanism during the development of human tumors.In this study,loss of heterozygosity (LOH)was investigated in 20cases of human gastric cancer and correspondent para- cancer samples using Southern blotting technique with the chromosomal 8 probe-D8S7.In 25% (5/20) of gastric carcinomas and 15% (3/20) of para-cancer samples were found to have lost an allele or had an allele deletion on chromosomal 8p.This finding suggests the presence of a tumor suppressor gene on short arm of chromosomal 8,which is involved in the development and progression of gastric carcinomas.