血小板衍生生长因子诱导人血管平滑肌细胞增殖时钙调素的变化

目的:观察血小板衍生生长因子(PDGF)对培养的人血管平滑肌细胞增殖及钙调素含量变化的作用.方法:应用3H-TdR掺入法和磷酸二酯酶法,观察不同浓度(1、10、20、30、40 ng/ml)PDGF-BB对培养的人血管平滑肌细胞DNA合成和钙调素含量变化的影响及其时间效应.结果:不同浓度PDGF-BB作用后,处于静止状态的人血管平滑肌细胞DNA的合成增加,并呈现明显的浓度依赖关系,在40 ng/ml浓度时DNA的合成达到高峰(P＜0.01).平滑肌细胞在进入细胞增殖周期后,细胞内钙调素含量逐渐增加,在9 h时出现一个短暂的高峰,随后逐渐下降;不同浓度PDGF-BB作用后,钙调素含量增加,并呈现出明显的浓度依赖关系,30 ng/ml浓度时作用最为显著(P＜0.01).结论:PDGF可促进培养的人血管平滑肌细胞增殖,该作用可能与增加钙调素含量有关.

Effects of platelet-derived growth factor BB on proliferation and calmodulin content in human vascular smooth muscle cells

Objective:To investigate the effects of platelet-derived growth factor BB(PDGF-BB)on proliferation and calmodulin content in human vascular smooth muscle cells(hVSMCs).Methods:The hVSMCs were cultured;the effects of PDGF-BB on DNA synthesis and calmodulin content in hVSMCs were assayed at different time points and different concentra-tions by 3 H-TdR incorporation and phosphodiesterase in vitro.Results:The DNA synthesis of quiescent hVSMCs was pro-moted in a dose-dependent manner,with the maximal response at a PGDF-BB concentration of40ng/ml at36h.Calmodulin level was increased after hVSMCs proliferation and reached a peak at9h.The content of calmodulin increased in a dose-de-pendent manner,with the maximal response at a PGDF-BB concentration of30ng/ml.Conclusion:PDGF-BB can promote the proliferation of hVSMCs,which may be related to the calmodulin level.