未经抗病毒治疗的慢性乙型肝炎病毒YMDD变异分析

目的探讨未经抗病毒治疗的慢性乙型肝炎患者中HBV多聚酶YMDD变异情况及与乙肝标志物之间的相关性。方法采用荧光定量PCR分析仪和ELISA方法分别对HBV-DNA含量、YMDD变异株及乙肝标志物进行检测。结果89例未经抗病毒治疗的慢性乙型肝炎患者YMDD变异率达13.5%,YMDD变异株以YIDD为主,占12.9%,YVDD占0.6%,并且其变异主要发生在HBeAg阳性患者中,HBeAg阳性占10.1%,抗-HBe阳性占3.4%。结论未经抗病毒治疗的乙肝患者仍存在较高的YMDD变异率,变异者中以YIDD变异株及集中在HBeAg阳性患者为主,临床上在应用抗病毒治疗前应对患者是否存在YMDD变异加以重视。

Analysis of YMDD Mutation in the no Antivirus-treated Chronic Hepatitis B

Objective To explore the relationship between YMDD mutation and the markers of HBV in the no antivirus-treated chronic hepatitis B.Methods The contents of HBV-DNA,YMDD mutants were detected by fluorescence quantitative polymerase chain reaction assay(FQ-PCR).The markers of HBV were detected by enzyme-linked immunoadsordent assay(ELISA).Results The rate of YMDD mutation was 13.5% in 89 chronic hepatitis B untreated by anti-virus,of which YIDD accounted for 12.9% and YVDD accounted for 0.6%,respectively.As to HBV markers,YMDD mutation mainly occurred in HBeAg positive patients.HBeAg positive rate was 10.1% and HBeAb positive rate was 3.4%.Conclusions Among chronic hepatitis B without anti-virus treatment,there was a highly YMDD mutation rate.Most mutants were YIDD mutant and occurred in HBeAg positive patients.