| 气相色谱(GC)法测定钩藤中钩藤碱的含量 |
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| 引用本文： | 高广涛,胡旭涛.气相色谱(GC)法测定钩藤中钩藤碱的含量[J].中医临床研究,2012,4(7):52-53. |
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| 作者姓名： | 高广涛 胡旭涛 |
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| 作者单位： | 漯河市郾城区人民医院,河南 漯河,462300 |
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| 摘 要： | 目的:建立气相色谱测定钩藤中钩藤碱含量的方法。方法:采用非极性毛细管柱AT.SE-54(15m×0.25mm×0.33μm);柱流速1.0ml.min-1;柱箱起始温度150℃,保持15min,以10℃.min-1的速率升至230℃,保持8min;进样口温度:280℃;检测器(FID)温度:280℃;载气为氮气。结果:钩藤碱在0.25442～1.2721μg范围内,线性关系良好(r=0.9998),平均回收率为99.21%,平均RSD为1.32%。结论:该方法稳定,结果可靠,重复性好,可作为可为钩藤质量控制标准的制定提供相应的依据。
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| 关 键 词： | 钩藤 钩藤碱 气相色谱法 |
Gas chromatography (GC) determination of the content of uncaria-rhynchophylline |
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| Abstract: | Objective:To establish the Gas chromatography(GC)determination of the content of Uncaria-rhynchophylline.Methods:A non-polar capillary column AT.SE-54(15m×0.25mm×0.33μm);The beginning of the column flow rate of 1.0 mL·min-1;Oven temperature of 150℃ for 15min,10℃.min-1.The rate rose to 230℃,to maintain 8min;Inlet temperature:280℃;detector(FID)temperature:280℃;Nitrogen as carrier gas.Results:rhynchophylline in the range of 0.25442～1.2721μg linear relationship(r = 0.9998),with an average recovery rate of 99.21%,the average RSD was 1.32%.Conclusion:This method is stable,reliable,reproducible,and can be used as for the of uncaria quality control standards to develop a corresponding basis. |
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| Keywords: | Uncaria Uncaria-rhynchophylline GC |
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