离心时间对凝血常规测定结果的影响

目的分析不同时间离心全血所得的血浆测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和纤维蛋白原浓度(FIB)的结果差异性,确定最适合实际工作的离心时间。方法将100例门诊患者的抗凝全血(每例两管)分为A组和B组,在相同条件下,分别离心10 min和5 min,用CA-7000型全自动血凝仪进行血凝常规检测,并且应用XE-2100全自动血细胞分析仪测定血小板计数。结果各项测定结果在离心5和10 min两组间差异无统计学意义(P>0.05);血浆血小板计数A组(离心10 min)和B组(离心5 min)的测定结果范围分别为(1～8)×109/L和(3～10)×109/L,均达到了乏血小板血浆的标准<(10～20)×109/L]1]。结论凝血常规标本可以3000 r/min离心5 min后进行测定。

The influence of centrifugal time to results of indexes of coagulation

Objective To ascertain the optimum centrifugal time for our work by analysing the differences between the blood plasma specimen＇s results which include plasma prothrombin time（ PT）, activated partial thrombo- plasting time（APTT） ,thrombin time （TT） and fibrinogen concentration（FIB） which are centrifuged in different time. Methods 100 outpatients vein entire blood samples （two per example） are divided into A group and B group ,which .is respectively centrifuged 10 minutes and 5 minutes in the same situation. The PT, APTT, TT and FIB results were tested by CA -7000 full-automatic blood coagulation analyzer. The platelct of the blood plasma were measured by Sysmex XE-2100 full-automatic blood cell analyzer. Results in centrifugal 10 minutes and 5 minutes has no significant difference （P 〉 0.05）. The range of the results of the platelet in the plasma for A group and the B group is（ 1 - 8） ×10^9/L and （3 - 10）×10^9/L respectively, both of them are up the standard of the poor platelet plasma 〈 （10-20） ×10^9/L ]. Conclusion Coagulation specimen can be measured by centrifuged with 3000 r/min speed,5 minutes.