实验性非压迫腰椎间盘髓核突出大鼠模型的建立

目的：设计一种新的非压迫性腰椎间盘髓核突出动物模型。方法：选择7只Sprague-Dawley(SD)雄性大鼠，将自身尾椎髓核混悬液注射到腰椎硬膜外腔建立动物模型。测量术前、术后3、5、7、9d大鼠后肢机械刺激缩爪阈值。结果：在没有明显机械压迫下，造模后大鼠出现明显的痛觉过敏反应。结论：本模型简单、可靠、费用低廉，为进一步研究腰椎间盘突出症和根性神经痛提供了理想的动物模型。

Establishment of Non- compressive Model Rat for Studying Lumbar Nucleus Pulposus prolapse - related Radiculalgia

Objective: Establishing non-compressive model rat with imitating lumbar nucleus pulposus prolapse to study the mechanism of nerve root pain. Methods: Seven SD rats were used. The suspension consisting of nucleus pulposus of coccygeal vertebra and normal saline was heteroautogenously transplanted into the cavum epiduralis of individual rat, creating a type of non-compressive model with nucleus pulposus autograft. The posterior limb's claws-retracting threshold for mechamical stimulation was determined shortly before and after operation on day 3 ,day 5, day 7 and day 9, respectively. Results: Without extrinsically overt mechanical compression of lumbar vertebrae, the murine model displayed hyperalgesic response. Conclusion: The established non-compressive model rat is an ideal and reliable method to study the mechanism of disc herniation-related radiculalgia.