X射线照射对人外周血淋巴细胞mIL2R表达的影响

目的观察Ｘ射线照射对人外周血淋巴细胞ｍＩＬ２Ｒ表达的影响。方法采用单克隆抗体间接免疫荧光标记ＦＣＭ检测。结果４Ｇｙ和６Ｇｙ单次照射组ｍＩＬ２Ｒ表达明显低于对照组（分别Ｐ＜０．０１）。７５ｍＧｙ及１００ｍＧｙ单次照射组ｍＩＬ２Ｒ表达明显高于对照组（分别Ｐ＜０．０１）。预先给予７５ｍＧｙ照射，继之又给予４Ｇｙ组ｍＩＬ２Ｒ表达明显高于单纯４Ｇｙ照射组。结论①４Ｇｙ以上Ｘ射线照射使ｍＩＬ２Ｒ的表达明显降低。②７５ｍＧｙ及１００ｍＧｙ小剂量照射后人外周血淋巴细胞ｍＩＬ２Ｒ表达显著增高。③７５ｍＧｙ预照射可诱导ｍＩＬ２Ｒ表达对其后４Ｇｙ照射的适应性反应。

Effect of X rays on mIL 2R expression of human lymphocytes

Objective The mIL 2R expression of human peripheral blood lymphocytes after X irradiation with different doses was investigated. [WTHZ]Method Indirect fluorescence analysis with flow cytometry was used. Results After 4 Gy or 6 Gy irradiation,the expression of mIL 2R was significantly lower than that of control (both P <0.01).However,the mIL 2R expression of lymphocytes was significantly higher than that of the control after irradiation with 75 mGy or 100 mGy ( P <0.001 and P <0.01,respectively).Furthermore, it was demonstrated that the mIL 2R expression in the group preirradiated with 75 mGy was higher than that in group irradiated with 4 Gy only. Conclusion 4 Gy or 6 Gy X irradiation reduced the mIL 2R expession of human peripheral blood lymphocytes,while mIL 2R expression was increased after irradiation with 75 mGy or 100 mGy;75 mGy preirradiation could induce adaptive response of mIL 2R expression.