丹参在缺血再灌注时的神经保护作用──成纤维细胞生长因子上调

丹参作为传统中药治疗缺血性脑血管病具有良好的疗效，本实验研究丹参是否通过影响成纤维细胞生长因子（ｂＦＧＦ）起修复作用。用大鼠线栓法缺血再灌注模型及免疫组化方法，发现大鼠缺血９０ｍｉｎ、再灌流４８ｈ后，缺血侧皮层、尾壳核及海马区ｂＦＧＦ样免疫反应增加及神经细胞变性；缺血后给予丹参（１５ｇ／　ｋｇ）大鼠，ｂＦＧＦ样免疫反应加强，并且缺血对应侧脑区也可见轻度的ｂＦＧＦ样免疫反应改变，且神经细胞变性程度较轻。提示丹参对脑缺血再灌流的保护作用可能与它加强ｂＦＧＦ的修复作用有关。

Protective Effects of RSM on Reperfusion Induced Cerebral Ischemia by Up-regulating bFGF

Background and purpose Radix Salviae Miltorrhizae(RSM)is a well known traditional Chinese medicine for treating ischemic cerebrovascular diseases and has been shown to attenuate ischemic damage to hippocampal CAl neurons in the gerbils. The purpose of the present study was to determine if RSM has any effect on basic fibroblast growth factors(bFGF)in ischemic rats.Methods Rats were exposed to left middle cerebral occlusion for 90 min, RSM was given(15 g/kg ip.)immediately after reperfusion. After 48 h reperfusion brains were examined for histological damage and the basic fibroblast growth factor(bFGF)expression using immunohistochemical technique.Results bFGF-like immunoreactivity was significantly higher in left cerebral cortex,caudate putamen and hippocampus, and neuronal damages in these brain regions was milder compared with controls.Conclusions The results indicate that RSM as judged by bFGF-like immunoreactivity and neuronal survival may help to attenuate ischemic cerebral injury induced by reperfusion.