聚合酶链反应用于恙虫病立克次体检测和分型的研究

本文报道聚合酶链反应用于恙虫病立克次本（Ｒｔ）检测和分型研究结果，以构建的Ｒｔ外膜主要蛋白５６ｋＤａ型特异抗原（ｔｓａ５６ｋＤａ）基因编码区的群型特异引物，采用１步法ＰＣＲ分别为Ｇｉｌｌｉａｎ，Ｋａｒｐ，Ｋａｔｏ，Ｋａｗａｓａｋｉ和Ｋｕｒｏｋｉ５株标准株Ｒｔ江苏地区５份Ｒｔ阳性标本，斑点热和莫氏立次体以及５份正常输血员全血标本进行检测和分型，研究结果证明所构建的Ｒｔ群，型引物具有Ｒｔ的特异性，型特

Studies on Detecting and Typing of Rickettsia Tsutsugamushi by polymerase Chain Rea ction

Institute qf Military Medicine, Nanjing Command PLA, Nanjing 210002One-step polymerase chain reaction (PCR) method was used for detecting and typing Rickettsia tsutsugamushi (Rt). The primers of the group and type were derived from the type-specific antigen (Tsa) gene DNA sequences (encodes the 56kDa antigen) of standard Rt, Gilliam, Karp, Kato, Kawasaki. Kuroki and Shimokoshi strains. These primers served to produce rickettsia-specific products in the amplification of templated DNA prepared from 6 strains standard Rt and 5 Positive Rt samples selected from Jiangsu Provinde 1No products in it fromRickettsia sPotted fever,Rickettsia mooseri and 5 blood donors. The typing primers pairs did not amplify crossly in types. The results indicated that these primer pairs were of the characteristic of Rt .it was useful for diagnosis,typing and investigating in epidemiology.The typing results indicated akain that the Rt discover belonged to a new serotype and dominant strain in epidemic in Jiangsu. This Rt was similar to Kawasaki strain of Japan. No another Rt strains were found in this study.