ACS患者体内同型半胱氨酸与黏附分子CD11b/CD18的相关性

目的:探讨急性冠脉综合征患者体内血浆同型半胱氨酸（HCY）与全血白细胞黏附分子CD11b/CD18水平的变化及其相关性。方法:对急性冠脉综合征（ACS）患者53例与健康人35例采用酶联免疫法测定血浆HCY水平,通过流式细胞仪采用直接免疫荧光法测定白细胞黏附分子CD11b/CD18的表达。结果:ACS组血浆HCY水平显著高于对照组,分别为29.1±13.5和12.7±6.9μmol/L（P<0.01）,ACS组白细胞表面CD11b/CD18表达水平显著高于对照组,分别为6.0±2.3比4.3±1.6 MFI（P<0.05）,27.7±7.7比19.6±4.9 MFI（P<0.05）。HCY水平与CD11b、CD18的表达显著相关。结论:同型半胱氨酸作为炎症介质可能通过调节白细胞黏附分子CD11b/CD18的表达,在ACS发生和发展中起重要作用。

The correlation between homocysteine level and CD11b/CD18 expression on leucocytes in the patients with acute coronary syndrome

AIM: To evaluate the plasma homocysteine level and CD11b/CD18 expression on leukocytes in whole blood and their relationship in patients with acute coronary syndrome (ACS). METHODS: Our study included 53 patients with ACS and 35 healthy controls based on their clinical situation, electrocardiogram, cardiac enzyme, cTNT and etc. HCY level was measured by enzyme immunoassay and leukocyte surface adhesive molecule CD11b/CD18 expressions were analyzed by immunofluorescence flow cytometry. RESULTS: The levels of plasma homocysteine were markedly increased and the expressions of CD11b/CD18 on leucocytes were significantly increased in patients with ACS compared to control group. The correlation between homocysteine level and CD11b/CD18 expression was positive in ACS. CONCLUSION: Homocysteine might play an important role in the occurrence of ACS by mediating expression of CD11b/CD18 on leukocyte as an inflammatory medium.