银杏叶提取物可抑制慢性阻塞性肺疾病大鼠气道及肺血管重塑

目的 探讨银杏叶提取物（GBE）对COPD大鼠气道及肺血管重塑的干预作用。方法 Wistar大鼠40只,随机分为正常对照组（A组）、造模不干预组（B组）、造模GBE早期干预组（C组）和造模GBE后期干预组（D组）。B、C和D三组采用慢性烟熏＋脂多糖气管内注射建立COPD模型,C和D组分别在1～14d和29～42d腹腔内注射GBE（0.40mL/kg）,43d后对各组大鼠肺脏行病理学检测。结果 B、C和D三组均有COPD特征性改变,但程度不同。A、B、C和D各组间肺泡平均面积、标准肺泡数比较均有显著差异（P均〈0.01）。C和D组管壁结构轻度改变,支气管平滑肌厚度/支气管壁厚度比值、支气管壁面积/支气管面积比值与A、B两组比较均有显著差异（P均〈0.01）。C和D组血管平滑肌细胞轻度增生,管壁轻度增厚,血管平滑肌细胞厚度/血管壁厚度比值、血管壁面积/血管面积比值与A、B两组比较均有显著差异（P均〈0.01）。结论 GBE对COPD大鼠模型气道重塑及肺血管重塑有抑制作用。

The inhibitory effects of ginkgo biloba extract on airway and vascular remodeling in rat model of chronic obstructive pulmonary disease

Objective To study the intervention effect of ginkgo biloba extract （GBE） on airway and vascular remodeling in rat model of chronic obstructive pulmonary disease （COPD）. Methods Forty wistar rats were randomly divided into group A, B, C and D. The rat model of COPD were established by intratracheally injection of lipopolysaccharide and exposure to cigarette smoke in groups B, C and D. Groups C and D were given intraperitoneally injection with 40 mg/kg GBE respectively from dayl to dayl4 and day29 to day42, Forty-three clays later, the rats were sacrificed for lung pathological examination, Results Group B, C and D all showed pathological changes characteristic of COPD to different extent. The average area and standard number of alveoli showed significant difference between each groups （ all P 〈 0. 01 ）, The structure of bronchiole walls in group C and D show mild changes. The ratio of bronchial smooth muscle thickness to bronchial wall thickness and bronchial wall area to bronchial area of group C and D showed significant difference when compared with group A and B （ all P 〈 0.01 ）, The vascular smooth muscle cell of group C and D had mild hyperplasia and the vascular wall had slightly thickened. The ratio of vascular smooth muscle thickness to vascular wall thickness and vascular wall area to vascular area in group C and D showed significant difference when compared with group A and B（ all P 〈 0.01 ）, Conclusion GBE has inhibitory effects on airway and vascular remodeling in rat model of COPD.