内侧隔区预毁损对海马CA1区迟发性神经元死亡的影响

目的 :探讨乙酰胆碱 (ACh)对短暂性全脑缺血再灌后海马CA1区迟发性神经元死亡 (DND)的作用。方法 :46只健康雄性Wistar大鼠随机分为 3组 :Ⅰ 手术对照组 ;Ⅱ MS假性损毁脑缺血再灌组 ;Ⅲ MS预损毁缺血再灌组 ,动物先行MS损毁 ,第 15天再行 4 VO ,使脑缺血 2 0min恢复灌注 72h后取材 ,采用NissleHE和TUNEL染色 ,在光镜下观测计数 ,经统计学处理。结果 :①Ⅱ、Ⅲ组缺血再灌后海马CA1出现迟发性神经元死亡 ;②Ⅲ组较Ⅱ组迟发性神经元损伤明显减轻。结论 :乙酰胆碱参与短暂性全脑缺血再灌后海马CA1区迟发性损伤过程 ,MS预毁损能使死亡细胞明显减少

The effect of MS lesion on delayed neuronal death in CA1 regions after global ischemia-reperfusion

Objective: To study the function of acetylcholine in the delayed neuronal death of CA1 pyramidal cells in a rat model of transient cerebral ischemia.Methods: The total of 46 rats were divided into three groups at random. Group Ⅰ, contrast group only operated on,but not suffered the lesion of medial septum; Group Ⅱ, the brain ischemia and reperfused, shamed the lesion of medial septum; Group Ⅲ, the brain ischemia and reperfused, suffered the lesion of medial septum. Rats firstly suffered the lesion of medial septum, then recovered for 15 days, secondly were subjected to the 4 vessel occlusion 20 minutes and reperfused 72 hours. The brains were regularly paraffin wax embedded,sectioned and stained by HE staining, TUNEL staining and immunohistochemical staining techniques. The neurons were observed, counted and evaluated under light microscope.Results: The DND occurred only in CA1 regions of group Ⅱ,Ⅲ, no obvious changes were observed in other regions of hippocampi. Compared with group Ⅱ, the labeled neurons of group Ⅲ was far fewer, the difference was significant ( P <0 05).Conclusion: Acetylcholine played an important role during the delayed neuronal death in CA1 subfield of hippocampi. The lesion of medial septum could alleviate the damage of CA1 neurons after ischemia