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Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands |
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| Authors: | Kragelund C Hansen C Torpet L A Nauntofte B Brøsen K Pedersen A M L Buchwald C Therkildsen M H Reibel J |
| Affiliation: | Division of Oral Medicine, Clinical Oral Physiology, Oral Pathology &Anatomy, Faculty of Health Sciences, Department of Odontology, University of Copenhagen, Copenhagen, Denmark;;Wilhelm Johansen Center for Functional Genome Research, Department of Medical Genetics, University of Copenhagen, Copenhagen, Denmark;;Centre of Clinical Pharmacology, University of Southern Denmark, Denmark;;Department of Otolaryngology, Head and Neck Surgery, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark;;Department of Pathology, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark |
| Abstract: | Objective: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands. Methods: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA. Results: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent. Conclusion: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva. |
| Keywords: | cytochrome P-450 salivary glands in situ hybridization immunohistochemistry |
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