大蒜素抑制体外人肝癌细胞的侵袭能力

Allicin inhibits invasion of human hepatocellular carcinoma cells in vitro

AIM: To investigate the influence of allicin on the ultramicromorphology of hepatocellular carcinoma cells, and to explore the genetic mechanism by which allicin inhibits the invasion and metastasis of hepatocellular carcinoma. METHODS: Human hepatocellular carcinoma BEL-7402 cells were cultured in the presence of adriamycin (5 mg/L), or allicin at low, medium and high concentrations(25, 50, and 100 mg/L, respectively). Metastasis-related ultrami-croscopic structures were examined 0.5 hour after drug treatment. Tumor invasion and metastasis inhibitory genes nm23-H1 and P21ras were assayed by flow cytometry 8 hours following treatment. The data were analyzed by X2 test with SAS 8.2 software. RESULTS: The growth of BEL-7402 cells was inhibited and apoptosis was induced following allicin treatment. Most of these adherent cells shrinked, with the junctions reduced, the intercellular space widened, and the cell surface microvilli decreased. Although cells treated with adriamycin also showed typical apoptosis morphology, there was no remarkable reduction of the microvilli on cell surface, and the cross junctions of microvilli among cells exhibited no changes either. The fluorescent intensities of the expression of nm23-H1 were 19.19 in negative control group, 119.76 in 5 mg/L adriamycin group, 84.8 in 25 mg/L allicin group, 92.64 50 mg/L allicin group, and 138.08 in 100 mg/L allicin group, respectively. Dose-effect relationship was clearly showed for allicin in the induction of nm23-H1 expression. The fluorescent intensity of 100 mg/L allicin group was remarkably stronger than that of 5 mg/L adriamycin group (138.08 vs 119.76, P<0.05). Negative or low expression of P21ras was observed in all groups, with no difference among them. CONCLUSION: Allicin can inhibit the invasion of hepato-cellular carcinoma cells, which may be caused by its influence on the motor system of hepatocellular carcinoma cells and its upregulation nm23-H1 gene expression.