水飞蓟宾对非酒精性脂肪性肝病线粒体膜流动性的影响

目的:探讨非酒精性脂肪性肝病(NAFLD)大鼠模型中肝脏线粒体膜流动性的改变以及观察水飞蓟宾对NAFLD的防治作用。方法:采用高脂饮食建立非酒精性脂肪性肝病大鼠模型,观察大鼠甘油三酸酯(TG)、胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)、肝组织HE染色、脂肪细胞染色的变化,以及肝脏线粒体膜的流动性的改变,并以水飞蓟宾抗氧化治疗,与已知有疗效的罗格列酮对比,观察其对上述指标的影响。结果:模型组血清ALT、AST、TG、TC显著升高,与空白对照组比较差异显著(P0.01)。HE染色提示肝组织呈弥散性脂质蓄积,模型组大鼠肝细胞线粒体微粘度明显高于空白对照组(P0.01)。罗格列酮治疗组TG、AST均得到明显改善(P0.05或P0.01);TC、ALT与模型组比较差异无显著(P0.05)。水飞蓟宾治疗后,TC、TG、ALT以及AST均得到明显改善(均P0.01)。两治疗组大鼠肝MDA含量、肝细胞线粒体微粘度均较模型对照组下降(P0.01),且水飞蓟宾的效果比罗格列酮显著,差异显著(P0.05)。结论:高脂饮食可诱导大鼠NAFLD发生,水飞蓟宾可以有效地防治NAFLD。稳定并维持适当的肝脏线粒体膜流动性、减轻肝脏脂质过氧化可能是水飞蓟宾肝脏保护功能的作用途径。

Effects of silybin on mitochondria membrane fluidity in rats with non-alcoholic fatty liver disease

AIM: To explore the effects of silybin on mitochondrial membrane fluidity in the rats with non-alcoholic fatty liver disease (NAFLD).METHODS: NAFLD rats were induced by high-fat diet for 6 weeks. Mitochondrial membrane fluidity, serum lipid levels, hepatic enzymes and peroxidant products were assayed. The histopathological changes of the livers were observed. The effects of silybin on the changes of the above parameters in NAFLD rats were also determined. Rosiglitazone was used as a positive control of treatment. RESULTS: Compared with the control animals, the fluidity of mitochondrial membrane was obviously decreased, and the contents of alanine aminotransferase, aspartate transaminase, triglyceride, total cholesterol, malonaldehyde and superoxide dismutase in serum were markedly increased (P<0.01) in the NAFLD rats. Silybin and rosiglitazone evidently regulated mitochondrial membrane fluidity, decreased the levels of serum lipids, improved the liver functions and meliorated histopathological manifestations of the livers (P<0.05 or P<0.01).CONCLUSION: High-fat diet successfully reproduces a NAFLD animal model. Stabilization of mitochondrial membrane and inhibition of oxidative stress may be the main mechanisms for the hepatoprotective effects of silybin in NAFLD.