Cross-bridge movement in rat slow skeletal muscle as a function of calcium concentration |
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Authors: | Hideyuki Honda Yoshiro Koiwa Naoto Yagi Ichiro Matsubara |
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Institution: | (1) The first Department of Internal Medicine, Tohoku University School of Medicine, 1-1 seiryo-machi, Aoba-ku, sendai, 980-77, Japan, JP;(2) The Department of Pharmacology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-77, Japan, JP |
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Abstract: | A single fibre bundle from rat soleus muscle was chemically skinned with saponin and the transfer of myosin heads from the
thick filaments to the thin filaments at a sarcomere length of 2.4 μm was measured as a function of Ca2+ concentration using an x-ray diffraction method at 4–7 °C. In the relaxed state, the 1,0 spacing was 42.08 nm. The spacing
showed no significant decrease when the Ca2+ concentration was below the threshold (−log10 Ca2+] or pCa 5.8). No significant transfer of the myosin heads occurred when the Ca2+concentration was below the threshold (pCa 5.8). When the muscle was maximally activated at pCa 4.4, the spacing decreased
to 40.35 nm. During the maximum isometric contraction at pCa 4.4, 54.9 ± 6.5% (±SE of the mean) of the myosin heads were transferred
to the thin filaments. The transfer of the myosin heads was approximately proportional to relative tension. These results
suggest that myosin heads of both fast-twitch and slow-twitch skeletal muscles transferred on the common movement as a function
of Ca2+ concentration.
Received: 1 December 1995/Received after revision and accepted: 20 May 1996 |
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Keywords: | Cross-bridge X-ray diffraction Slow-twitch skeletal muscle |
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