| 龙胆泻肝丸中龙胆苦苷含量的检测方法 |
| |
| 引用本文： | 杨务彬,李元宏,兰鸿.龙胆泻肝丸中龙胆苦苷含量的检测方法[J].中国医药指南,2009,7(18):28-30. |
| |
| 作者姓名： | 杨务彬 李元宏 兰鸿 |
| |
| 作者单位： | 湖北省十堰太和医院药学部,442000 |
| |
| 摘 要： | 目的建立龙胆泻肝丸中龙胆苦苷含量测定的方法。方法色谱柱:VP-ODS柱(150mm×4.6mm,5μm);流动相:A组分:乙腈:0.1%磷酸溶液(10∶90);检测波长270nm;流速:1.0mL/min;柱温:25℃。结果龙胆苦苷的线性范围是0.3554～5.331μg,r=0.9998,平均回收率是100.04%,RSD=1.80%。结论该法可同时测定龙胆泻肝丸中龙胆苦苷和栀子苷的含量。
|
| 关 键 词： | 龙胆泻肝丸 龙胆苦苷 高效液相色谱法 含量测定 |
A Method of the Content Determination of Gentiopicroside in Longdan Xiegan Pill |
| |
| YANG Wu-bin,LI Yuan-hong,LAN Hong.A Method of the Content Determination of Gentiopicroside in Longdan Xiegan Pill[J].Guide of China Medicine,2009,7(18):28-30. |
| |
| Authors: | YANG Wu-bin LI Yuan-hong LAN Hong |
| |
| Institution: | (Department of Pharmacy, Shiyan Taihe Hospital, Shiyan 442000, China) |
| |
| Abstract: | Objective To establish a HPLC method for determination of Gentiopicroside in Longdan Xiegan Pill. Methods The analysis was carried on an analytical column VP-ODS（150mm×4.6mm）. The mobile phase consisted of acetonitrile： 0. 1%phosphoric acid （10 ： 90） and the flow rate was 1.0mL/min. The UV dection wave length was at 270nm and column temperature was 25 ℃. Results The liner range of Gentiopicroside was 0.3554-5.331 μg （r =0.9998） and the average recovery 100.04%（RSD=1.80%, n=6）. Conclusions The methods is simple, reproducible and suitable for determination of Gentiopicroside in Longdan Xiegan Pill with good chromatographic separation. |
| |
| Keywords: | Longdan Xiegan Pill Gentiopicroside High efficiency liquid chromatography Content determination |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
