补钙对骨质疏松并发骨折大鼠的生物力学研究

目的探讨补钙对骨质疏松并发骨折的大鼠模型生物力学的影响。方法将105只6月龄 Sprague-Dawley大鼠随机分5组:假切组(Sham)、去势组(OVX)、去势 L-苏糖酸钙高剂组、去势 L-苏糖酸钙中剂组、去势 L-苏糖酸钙低剂组。在去势手术后的第20d末(给药13d末)和第40d末(给药33d末)处死动物。取左前肢分别进行X光片拍摄、组织学切片的观察和三点弯曲生物力学试验测试。结果 X光片、组织学切片的观察都显示了正常对照组与模型对照组之间有明显的放射学和形态学改变;且给予L-苏糖酸钙不同剂量的钙剂后,骨折部位有不同程度愈合的放射学和形态学改变,尤以中剂量组最为显著。三点弯曲生物力学试验结果显示:给药13d和给药 33d后,假切组的最大桡度、最大弯曲力和斜率与去势组相比均有显著差异(P<0．01或P< 0．05);L-苏糖酸钙高、中、低剂组也与去势组相比有显著差异(P<0．01)。给药33d后,L-苏糖酸钙高(P<0．01)、中、低剂组和Sham组(P<0．05)的最大弯曲力与给药13d相比有显著差异; L-苏糖酸钙中剂组的斜率与给药13d相比也有显著差异(P<0．01)。结论适量的钙剂能显著促进骨折愈合,改善骨痂质量,增强骨生物力学特性。

Biomechanics study of calcium supplementation on osteoporosis with fracture

Objective To study the effect of calcium supplementation on ovariectomized rats with fracture. Methods One hundred and five six-month-old SD rats were randomly divided into 5 groups: sham, ovx, ovx L-threose acid calcium of 200mg Ca/kg/day, ovx L-threose acid calcium of 100mg Ca/kg/day, and ovx L-threose acid calcium of 50mg Ca/kg/day groups. All rats were sacrificed at the end of 20 days after ovariectomy (13 days after therapy) and 40 days after ovariectomy (33 days after therapy) respectively. Left forelimb was carried out and tested by X-ray, histology and three-point-bending test. Results Both of the control and model control group presented radiologic and morphological changes which in different extend in different groups. The most remarkable changes was shown in the 100 mg Ca/kg/d group. Conclusions Proper calcium supplementation can fasten the fracture healing process, improve the quality of callus and enhance the biomechanical properties.