抗禽流感病毒H5N1亚型单克隆抗体制备初报

为建立诱发动脉粥样硬化斑块破裂的动物模型，选用雄性新西兰兔，给予高脂饮食2周，利用球囊导管去除家兔腹主动脉内皮，继以高脂饮食至8周，正常饮食至16周即实验末，腹腔内注射鲁塞尔氏蝰蛇毒和静脉注射组胺诱发斑块破裂和血栓形成，利用图像分析法计算血栓形成面积，光镜和电镜观察腹主动脉或斑块的形态学表现，酶法测定兔血脂和血浆脂蛋白水平以及腹主动脉壁中胆固醇含量。结果表明，模型组家兔腹主动脉有大量血栓形成，显微镜下可见斑块肩部区有明显破溃和炎性细胞浸润，斑块内富含巨噬细胞和脂滴，在实验2、8和16周时的血清TC、LDL-C水平均显著升高，腹主动脉壁中胆固醇含量也远高于正常组。结果显示，这些改变符合不稳定斑块的特征，提示该模型可用于斑块破裂发生机制和筛选稳定斑块药物的研究。

Preparation of Monoclonal Antibodies Specific to Subtype H5N1 of Avian Influenza Virus

Objective To prepare the monoclonal antibodies specific to subtype of H5N1 avian influenza virus and offer tool for correlative researches.Methods Monoclonal antibodies specific to subtype of H5N1 avian influenza virus were developed by fusing SP2/0 myeloma cell with spleen cell of BALB/c mice immunized with AIV_H5N1, the characterization of three McAbs can specifically react with AIV_H5N1 by hemagglutination inhibit test(HI), enzyme linked immunosorbent assay(ELISA) and immunofluorescence. The inmmunoglobulin subclass of the McAbs was then identified. Results Three colone hybridoma secreting McAbs against AIV_H5N1 were obstained as F8,F9,G11 respectively, the inmmunoglobulin subclass of the McAbs was identified as IgG1,IgG2a and IgG2b. Conclusion The highly effective and specific McAbs G11 can play an important role in diagnosis, treatment and prevention of the infection of AIV_H5N1