中药天年饮对衰老大鼠脑天门冬氨酸含量的影响

目的：观察中药天年饮对衰老大鼠不同脑区兴奋性氨基酸天门冬氨酸（aspartic acid，Asp）含量的影响。方法：40只雌性SD大鼠随机分为4组，正常组、衰老模型组、天年饮用药组和阴性对照组，每组均为10只。D-半乳糖连续腹腔注射建立亚急性衰老的大鼠模型。采用高效毛细管电泳法检测各组大鼠大脑皮质、海马、下丘脑Asp的含量。结果：衰老大鼠皮质、海马、下丘脑Asp的含量均明显高于衰老模型大鼠（P〈0．01，P〈0．05）；中药天年饮可明显降低衰老大鼠皮质、海马、下丘脑Asp的含量（用药组与衰老模型组比较：P〈0．01，P〈0．05）。结论：中药天年饮可抑制衰老时Asp的异常释放，改善神经元的功能。

EFFECTS OF TRADITIONAL CHINESE MEDICINE TIANNIANYIN ON CONTENT OF ASPARTIC ACID IN BRAIN OF AGING RATS

Objective：To observe the effects of traditional Chinese medicine tiannianyin on content of aspartic acid （Asp） in different brain region of aging rats.Methods：40 female SD rats were randomly divided into 4 groups：normal group, aging model group,tiannianyin group and negative control group,each group had 10 rats.The sub-acutely aging model rats were made by injecting D-gal into abdominal cavity continually.High performance capillary electropheresis （HPCE） was used to detect the contents of Asp in cerebral cortex,hippocampus and hypothalamus of rats in each group.Results：The content of Asp in cerebral cortex,hippocampus and hypothalamus of aging rats were obviously higher than that of normal rats （P 〈 0.01, P 〈 0.05）.Traditional Chinese medicine tiannianyin could obviously decrease the Asp content in cerebral cortex, hippocampus and hypothalamus of aging rats （tiannianyin group compared with aging model group：P 〈 0.01,P 〈 0.05）.Conclusions： Traditional Chinese medicine tiannianyin can inhibit abnormal secretion of Asp during aging,so improve the function of neurons.