| Dendritic Cell-Derived Exosomes Stimulate Stronger CD8~+ CTL Responses and Antitumor Immunity than TVimor Cell-Derived Exosomes |
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| 引用本文： | Mabood Qureshi,Jim Xiang. Dendritic Cell-Derived Exosomes Stimulate Stronger CD8~+ CTL Responses and Antitumor Immunity than TVimor Cell-Derived Exosomes[J]. Cellular & molecular immunology, 2006, 0(3) |
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| 作者姓名： | Mabood Qureshi Jim Xiang |
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| 作者单位： | Research Unit Division of Health Research，Saskatchewan Cancer Agency，Departments of Oncology，Immunology and Pathology，College of Medicine，University of Saskatchewan，Saskatoon，Saskatchewan S7N 4H4，Canada，Research Unit，Division of Health Research，Saskatchewan Cancer Agency，Departments of Oncology，Immunology and Pathology，College of Medicine，University of Saskatchewan，Saskatoon，Saskatchewan S7N 4H4，Canada |
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| 摘 要： | Exosomes (EXO) derived from dendritic cells (DC) and tumor cells have been used to stimulate antitumor immune responses in animal models and in clinical trials. However, there has been no side-by-side comparison of the stimulatory efficiency of the antitumor immune responses induced by these two commonly used EXO vaccines. In this study, we selected to study the phenotype characteristics of EXO derived from a transfected EG7 tumor cells expressing ovalbumin (OVA) and OVA-pulsed DC by flow cytometry. We compared the stimulatory effect in induction of OVA-specific immune responses between these two types of EXO. We found that OVA protein-pulsed DCovA-derived EXO (EXODC) can more efficiently stimulate naive OVA-specific CD8+ T cell proliferation and differentiation into cytotoxic T lymphocytes in vivo, and induce more efficient antitumor immunity than EG7 tumor cell-derived EXO (EXOEG7). In addition, we elucidated the important role of the host DC in EXO vaccines that the stimulatory effect of EXO is delivered to T cell responses by the host DC. Therefore, DC-derived EXO may represent a more effective EXO-based vaccine in induction of antitumor immunity.
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Dendritic Cell-Derived Exosomes Stimulate Stronger CD8~+ CTL Responses and Antitumor Immunity than TVimor Cell-Derived Exosomes |
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| Siguo Hao,Ou Bai,Jinying Yuan,Mabood Qureshi and Jim Xiang Research Unit,Division of Health Research,Saskatchewan Cancer Agency,Departments of Oncology,Immunology and Pathology,College of Medicine,University of Saskatchewan,Saskatoon,Saskatchewan SN H,Canada. Dendritic Cell-Derived Exosomes Stimulate Stronger CD8~+ CTL Responses and Antitumor Immunity than TVimor Cell-Derived Exosomes[J]. Cellular & molecular immunology, 2006, 0(3) |
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| Authors: | Siguo Hao Ou Bai Jinying Yuan Mabood Qureshi Jim Xiang Research Unit Division of Health Research Saskatchewan Cancer Agency Departments of Oncology Immunology Pathology College of Medicine University of Saskatchewan Saskatoon Saskatchewan SN H Canada |
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| Affiliation: | Siguo Hao,Ou Bai,Jinying Yuan,Mabood Qureshi and Jim Xiang Research Unit,Division of Health Research,Saskatchewan Cancer Agency,Departments of Oncology,Immunology and Pathology,College of Medicine,University of Saskatchewan,Saskatoon,Saskatchewan S7N 4H4,Canada |
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| Abstract: | Exosomes (EXO) derived from dendritic cells (DC) and tumor cells have been used to stimulate antitumor immune responses in animal models and in clinical trials. However, there has been no side-by-side comparison of the stimulatory efficiency of the antitumor immune responses induced by these two commonly used EXO vaccines. In this study, we selected to study the phenotype characteristics of EXO derived from a transfected EG7 tumor cells expressing ovalbumin (OVA) and OVA-pulsed DC by flow cytometry. We compared the stimulatory effect in induction of OVA-specific immune responses between these two types of EXO. We found that OVA protein-pulsed DCovA-derived EXO (EXODC) can more efficiently stimulate naive OVA-specific CD8+ T cell proliferation and differentiation into cytotoxic T lymphocytes in vivo, and induce more efficient antitumor immunity than EG7 tumor cell-derived EXO (EXOEG7). In addition, we elucidated the important role of the host DC in EXO vaccines that the stimulatory effect of EXO is delivered to T cell responses by the host DC. Therefore, DC-derived EXO may represent a more effective EXO-based vaccine in induction of antitumor immunity. |
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| Keywords: | dendritic cell exosome tumor cell cytotoxic T lymphocyte antitumor immunity |
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