Measuring receptor/ligand interaction at the single-bond level: experimental and interpretative issues |
| |
| Authors: | Zhu Cheng Long Mian Chesla Scott E Bongrand Pierre |
| |
| Affiliation: | (1) Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA;(2) Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA;(3) College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China;(4) National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Science, Beijing, 100080, People's Republic of China;(5) Immucor, Inc., Norcross, GA;(6) INSERM U 387, Laboratoire d'Immunologie, Hopital de Sainte-Marguerite, BP 29, 13274 Marseille Cedex 09, France |
| |
| Abstract: | There is increased interest in measuring kinetic rates, lifetimes, and rupture forces of single receptor/ligand bonds. Valuable insights have been obtained from previous experiments attempting such measurements. However, it remains difficult to know with sufficient certainty that single bonds were indeed measured. Using exemplifying data, evidence supporting single-bond observation is examined and caveats in the experimental design and data interpretation are identified. Critical issues preventing definitive proof and disproof of single-bond observation include complex binding schemes, multimeric interactions, clustering, and heterogeneous surfaces. It is concluded that no single criterion is sufficient to ensure that single bonds are actually observed. However, a cumulative body of evidence may provide reasonable confidence. © 2002 Biomedical Engineering Society.PAC2002: 8715Kg, 8715By, 8716-b |
| |
| Keywords: | Sensitive force techniques Probabilistic model Adhesion likelihood Attachment lifetime Rupture force |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
