维生素E对低温暴露大鼠肝线粒体功能的影响

1.本实验对动物在低温环境中补充维生素E的摄取量,对肝线粒体功能的影响进行了观察。 2.在-2°±1℃的环境用基础饲料补充30mg维生素E/100g饲养大鼠,14天后,离休肝线粒体ADP/○比值下降显著减少,RCR降低显著改善,说明在低温环境中增加维生素E的摄取量对肝线粒体的功能有保护作用。 3.本丈对低温环境中补充维生素E对机体耐寒能力的影响作了讨论。

THE EFFECT OF VITAMIN E ON THE MITOCHONDRIAL FUNCTION OF COLD EXPOSED RATS

To evaluate the effect of Vitamin E on mitoc bond rial function of cold exposed rats male weaning rats were divided randomly into three groups. The group A was supplemented with 30 mg α-tocopherol and 15μg sodium selenite per 100gm basal diet. The group B and C were fed basal diet on- ly. The group A and B were kept at the cold room (-2±1℃) and the group C kept at room temperature. Liver mitochondria were isolated for measurement of its respiratory function. Succinate was utilized as substrates of oxidative phosphorylation. Respiration rates of state 3 oxygen uptake and therefore ATP synthesis were found to increase with Vitamin E supplemented diet. Significantly less decline of ADP/O ratios and RCR was observed for group A than that of group B. These results suggest that Vitamin E has the effect of maintaining the physiological intactness of mitochondria of the cold exposed rats, and therefore may enhance the metabolic conservation of energy with the consequence of increasing the ability of cold tolerance of the cold exposed animals.