半乳糖凝集素-3在病毒性心肌炎小鼠心肌组织中的动态变化及临床意义

目的 探讨半乳糖凝集素-3(Gal-3)在小鼠柯萨奇病毒B3(CVB3)所致病毒性心肌炎(VMC)中的表达及其临床意义.方法 选取58只健康雄性Balb/C小鼠,随机分为VMC组(n=50)和对照组(n=8).VMC组小鼠腹腔接种半数组织培养感染剂量( TCID50)为10-5 L-1的Nancy株CVB3病毒液0.1mL,分别于接种第7、10、14、28天随机取8只处死；对照组小鼠腹腔接种不含病毒的eagle氏液0.1 mL,于第28天处死.光镜下观察小鼠心肌组织的病理变化并计算心肌病理积分；运用实时荧光定量PCR检测心肌组织中Gal-3 mRNA的表达,应用ELISA法检测外周血Gal-3水平,并与心肌病理积分作直线相关性分析.结果 1.与对照组小鼠比较,VMC组小鼠心肌组织Gal-3 mRNA的表达在第7天(0.91 ±0.64 vs 10.09 ±2.78)、第10天(0.91 ±0.64 vs 12.49 ±6.08)和第14天(0.91 ±0.64vs2.86±2.90)均明显增高(Pa＜0.05).2.与对照组小鼠外周Gal-3水平(5.27±1.11)比较,VMC组小鼠外周血Gal-3水平在第7天(9.70±2.39)、第10天(8.98±3.03)和第14天(7.73±1.88)也均明显增高(Pa＜0.05).3.小鼠心肌组织中Gal-3 mRNA与外周血中Gal-3表达水平均与心肌病理积分呈直线正相关(r=0.77、0.69,Pa＜0.05).结论 Gal-3参与VMC的发生与发展,且与VMC的病情严重程度有关,可作为VMC新的检测指标.

Dynamic Change of Galectin -3 in Myocardium Tissue of Mice with Viral Myocarditis and Its Clinical Significance

Objective To investigate the dynamic change of Galectin-3(Gal-3) in mice with coxsackie virus B3(CVB3) induced myocarditis and its clinical significance in viral myocarditis(VMC).Methods A total of 58 healthy male Balb/C mice were randomly divided into VMC group(n=50) and control group(n=8).The mice in VMC group were treated with 0.1 mL 10-5 L-1 CVB3,and 8 cases of infected mice were respectively sacrificed on the 7th day,10th day,14th day and 28th day;while the mice in control group were inoculated intraperitoneally with 0.1 mL eagle reagent and sacrificed on the 28th day.The myocardial pathological changes were observed through light microscope and histopathological scores were calculated.In addition,the expressions of Gal-3 in myocardial and serum were respectively detected by means of real-time quantitative-polymerase chain reaction and enzyme-linked immunosorbent assay,and then linear correlation analysis with myocardial histopathology scores was conducted.Results 1.Compared with control group,the expression of Gal-3 mRNA in myocardial in VMC group obviously increased on the 7th day(0.91±0.64 vs 10.09±2.78,P<0.05),10th day(0.91±0.64 vs 12.49±6.08,P<0.05) and 14th day(0.91±0.64 vs 2.86±2.90,P<0.05).2.The serum concentration of Gal-3 in VMC group obviously increased on the 7th day(9.70±2.39),10th day(8.98±3.03) and 14th day(7.73±1.88) compared with control group(5.27±1.11)(Pa<0.05).3.There were significantly positive correlations between the expression of Gal-3 mRNA in myocardial and serum with myocardial histopathological scores respectively(r=0.77,0.69;Pa<0.05).Conclusions Gal-3 could be involved in the pathogenesis and development of VMC and related with the extent of VMC.It might serve as a new detection mark for VMC.