| TrkA、p75NTR mRNA在肛门直肠畸形胎鼠直肠末端的表达 |
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| 引用本文： | 毛羽晨,刘远梅,孔萌,金祝,高明娟.TrkA、p75NTR mRNA在肛门直肠畸形胎鼠直肠末端的表达[J].实用儿科临床杂志,2012,27(11):826-828. |
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| 作者姓名： | 毛羽晨 刘远梅 孔萌 金祝 高明娟 |
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| 作者单位： | 遵义医学院附属医院小儿普胸泌外科,贵州遵义,563003 |
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| 基金项目： | 贵州省科技厅社发公关项目 |
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| 摘 要： | 目的通过检测肛门直肠畸形(ARM)胎鼠直肠末端神经生长因子受体TrkA、p75NTR mRNA的表达水平,探讨ARM直肠末端肠神经系统发育情况。方法成年SD孕鼠16只。其中实验组10只,对照组6只。实验组孕鼠在孕11 d、13 d 2次给予10 g.L-1乙烯硫脲(125 mg.kg-1)灌胃,制作先天性ARM动物模型;对照组灌胃注入等量9 g.L-1盐水。2组孕鼠在孕20 d时行剖宫手术取出胎鼠,取其直肠末端并保存于-80℃冰箱;采用反转录/实时定量PCR(RT-PCR)方法检测正常胎鼠和ARM胎鼠直肠末端TrkA、p75NTR mRNA表达情况。结果 TrkA、p75NTR mRNA在正常胎鼠直肠末端的相对表达量分别是162.221±104.675,129.778±51.976,在ARM胎鼠直肠末端中表达分别是78.937±33.425,87.145±25.812,两组比较差异均有统计学意义(Pa<0.05);琼脂糖凝胶电泳显示TrkA、p75NTR基因扩增后产物cDNA片段与引物设计的扩增片段完全吻合,TrkA、p75NTR基因条带亮度对照组均高于实验组。结论 TrkA、p75NTR mRNA在ARM胎鼠直肠末端的表达异常,提示神经生长因子及受体TrkA、p75NTR可能参与ARM肠神经系统的发育。
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| 关 键 词： | TrkA p75NTR 肛门直肠畸形 肠神经系统 |
Expressions of TrkA and p75NTR mRNA in Distal Rectum of Fetal Rats with Anorectal Malformations |
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| MAO Yu-chen
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LIU Yuan-mei
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KONG Meng
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JIN Zhu
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GAO Ming-juan.Expressions of TrkA and p75NTR mRNA in Distal Rectum of Fetal Rats with Anorectal Malformations[J].Journal of Applied Clinical Pediatrics,2012,27(11):826-828. |
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| Authors: | MAO Yu-chen LIU Yuan-mei KONG Meng JIN Zhu GAO Ming-juan |
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| Institution: | (Department of General & Thoracic & Urology Pediatric Surgery,the Hospital Affiliated to Zunyi Medical College,Zunyi 563003,Guizhou Pro-vince,China) |
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| Abstract: | |
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| Keywords: | TrkA p75NTR anorectal malformation enteric nervous system |
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